Drummond:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Local Protocols)
Current revision (09:47, 13 October 2012) (view source)
(rather rapid alternative)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
{{Drummond_Top}}
{{Drummond_Top}}
<div style="width: 750px">
<div style="width: 750px">
 +
__NOTOC__
==Local Protocols==
==Local Protocols==
Here are some protocols we use in the lab.
Here are some protocols we use in the lab.
 +
*[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]]
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]]
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]]
Line 11: Line 13:
*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]
*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]
*[[Drummond:Transformation|High-efficiency yeast transformation]]
*[[Drummond:Transformation|High-efficiency yeast transformation]]
-
*[[Drummond:DNA Prep|Yeast genomic DNA prep]]
+
*[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead)
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]]
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]]
Line 20: Line 22:
==Remote Protocols==
==Remote Protocols==
*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]
*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]
-
*[http://www.fhcrc.org/science/labs/gottschling/yeast/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]
+
*[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]

Current revision

the drummond lab

home      people      research      publications      news      jobs      protocols      contact     


Local Protocols

Here are some protocols we use in the lab.

Remote Protocols

Data Analysis Protocols

Notes

Personal tools