Drummond:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Dadrummond (talk | contribs) (rather rapid alternative) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Drummond_Top}} | {{Drummond_Top}} | ||
<div style="width: 750px"> | <div style="width: 750px"> | ||
__NOTOC__ | |||
==Local Protocols== | ==Local Protocols== | ||
Here are some protocols we use in the lab. | Here are some protocols we use in the lab. | ||
*[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]] | |||
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | ||
| Line 11: | Line 13: | ||
*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]] | *[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]] | ||
*[[Drummond:Transformation|High-efficiency yeast transformation]] | *[[Drummond:Transformation|High-efficiency yeast transformation]] | ||
*[[Drummond:DNA Prep|Yeast genomic DNA prep]] | *[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead) | ||
*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]] | *[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]] | ||
*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | *[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | ||
| Line 20: | Line 22: | ||
==Remote Protocols== | ==Remote Protocols== | ||
*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)] | *[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)] | ||
*[http:// | *[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)] | ||
*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | *[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | ||
Latest revision as of 07:47, 13 October 2012
Local Protocols
Here are some protocols we use in the lab.
- Chloroform/methanol precipitation of proteins
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- Standard conditions for flow cytometer growth rate assay
- High-efficiency yeast transformation
- Yeast genomic DNA prep (consider the rather rapid yeast genomic DNA prep instead)
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
