Drummond:Protocols: Difference between revisions
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*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | ||
*[[Drummond:Fluorescence|Cell preparation and protocol to view RFP/TFP fluorescence]] | |||
==Remote Protocols== | ==Remote Protocols== |
Revision as of 12:25, 20 June 2008
Local Protocols
Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
Remote Protocols
Rather rapid yeast genomic DNA prep (Gottschling Lab)
SDS-PAGE (Szostak Lab)