Drummond:Protocols: Difference between revisions
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==Data Analysis Protocols== | ==Data Analysis Protocols== | ||
*[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | *[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | ||
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Revision as of 20:09, 23 January 2009
Local Protocols
Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High Efficiency Yeast Transformation
- Yeast Genomic DNA Prep
- Isolating Total, Soluble and Insoluble Protein Fractions
- The relationship between the selection coefficient and relative growth rates: under construction
