Drummond:Protocols: Difference between revisions
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*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | *[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | ||
*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]] | *[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]] | ||
*[[Drummond:Periplasmic Prep|Periplasmic protein prep from <i>E. coli</i>]] | |||
<!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --> | <!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --> | ||
Revision as of 16:27, 27 March 2009
Local Protocols
Here are some protocols we use in the lab.
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
