Drummond:Protocols
From OpenWetWare
Local Protocols
Here are some protocols we use in the lab.
- Extracting soluble and insoluble protein fractions
- Thoughts on lysis of yeast cells
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High Efficiency Yeast Transformation
- Yeast Genomic DNA Prep
- Isolating Total, Soluble and Insoluble Protein Fractions
- The relationship between the selection coefficient and relative growth rates: under construction