http://www.openwetware.org/index.php?title=Drummond:Protocols&feed=atom&action=history 2013-05-19T06:50:40Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=635063&oldid=prev 2012-10-13T14:47:10Z

<p>rather rapid alternative</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:47, 13 October 2012</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]] <ins class="diffchange diffchange-inline">(consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=565247&oldid=prev 2011-11-08T18:38:03Z

<p>no toc</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:38, 8 November 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Drummond_Top}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Drummond_Top}}</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;div style=&quot;width: 750px&quot;&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;div style=&quot;width: 750px&quot;&gt;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">__NOTOC__</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Local Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Local Protocols==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here are some protocols we use in the lab.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here are some protocols we use in the lab.</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=537636&oldid=prev 2011-09-15T02:26:00Z

<p><span class="autocomment">Remote Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 02:26, 15 September 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 21:</td> <td colspan="2" class="diff-lineno">Line 21:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Remote Protocols==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Remote Protocols==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[http://<del class="diffchange diffchange-inline">www</del>.fhcrc.org<del class="diffchange diffchange-inline">/science/labs</del>/gottschling/<del class="diffchange diffchange-inline">yeast</del>/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[http://<ins class="diffchange diffchange-inline">labs</ins>.fhcrc.org/gottschling/<ins class="diffchange diffchange-inline">Yeast%20Protocols</ins>/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=523497&oldid=prev 2011-07-15T14:00:31Z

<p><span class="autocomment">Local Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:00, 15 July 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 4:</td> <td colspan="2" class="diff-lineno">Line 4:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here are some protocols we use in the lab.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here are some protocols we use in the lab.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=305440&oldid=prev 2009-05-01T18:46:29Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:46, 1 May 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth <del class="diffchange diffchange-inline">before flow cytometer fitness assay</del>| Standard conditions for flow cytometer growth rate assay]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Kerry A Geiler http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=305439&oldid=prev 2009-05-01T18:45:45Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:45, 1 May 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth <ins class="diffchange diffchange-inline">before flow cytometer fitness assay</ins>| Standard conditions for flow cytometer growth rate assay]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Kerry A Geiler http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=305266&oldid=prev 2009-04-30T21:47:26Z

<p><span class="autocomment">Local Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:47, 30 April 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth conditions for flow cytometer growth rate assay]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Drummond:Pregrowth<ins class="diffchange diffchange-inline">| Standard </ins>conditions for flow cytometer growth rate assay]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Kerry A Geiler http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=305265&oldid=prev 2009-04-30T21:46:25Z

<p><span class="autocomment">Local Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:46, 30 April 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Viability|Yeast OD and cell viability model]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Drummond:Pregrowth conditions for flow cytometer growth rate assay]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Transformation|High-efficiency yeast transformation]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:DNA Prep|Yeast genomic DNA prep]]</div></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Kerry A Geiler http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=297283&oldid=prev 2009-03-27T23:27:12Z

<p><span class="autocomment">Local Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:27, 27 March 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Drummond:Periplasmic Prep|Periplasmic protein prep from &lt;i&gt;E. coli&lt;/i&gt;]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond http://www.openwetware.org/index.php?title=Drummond:Protocols&diff=295420&oldid=prev 2009-03-20T23:41:40Z

<p><span class="autocomment">Local Protocols</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:41, 20 March 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Drummond:Competent Cells|Making chemically competent &lt;i&gt;E. coli&lt;/i&gt; cells]]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --&gt;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>&lt;!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --&gt;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-19 06:50:40 --> </table>

Dadrummond