E. coli genotypes - Revision history

Janet B. Matsen: /* DH10B (Invitrogen) */

Janet B. Matsen — Wed, 13 Mar 2013 13:59:18 GMT

DH10B (Invitrogen)

←Older revision		Revision as of 13:59, 13 March 2013
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	*Genome sequence indicates that DH10B is actually deoR<sup>+</sup>. Presumably TOP10 and MC1061 are also deoR<sup>+</sup>.		*Genome sequence indicates that DH10B is actually deoR<sup>+</sup>. Presumably TOP10 and MC1061 are also deoR<sup>+</sup>.
	*Streptomycin resistant		*Streptomycin resistant
		+	*leucine auxotroph
	*References:		*References:
	**Casdaban, M. and Cohen, S. (1980) J Mol Biol 138:179 PMID 6997493.		**Casdaban, M. and Cohen, S. (1980) J Mol Biol 138:179 PMID 6997493.

Janet B. Matsen: /* TOP10 (Invitrogen) */

Janet B. Matsen — Wed, 13 Mar 2013 13:56:56 GMT

TOP10 (Invitrogen)

←Older revision		Revision as of 13:56, 13 March 2013
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	** φ80lacZΔM15 actually contains the entire lac operon, including lacI<sup>q</sup> --[[User:Dcekiert\|Dcekiert]] 16:45, 23 January 2008 (CST)		** φ80lacZΔM15 actually contains the entire lac operon, including lacI<sup>q</sup> --[[User:Dcekiert\|Dcekiert]] 16:45, 23 January 2008 (CST)
	***Analysis of the published DH10B sequence (Genbank CP000948) suggests the φ80lacZΔM15 insertion has the wild-type lacI -35 sequence, not the lacI<sup>q</sup> -35 sequence (gtgcaa) --[[User:Barry Canton\|BC]] 15:01, 29 March 2008 (EDT)		***Analysis of the published DH10B sequence (Genbank CP000948) suggests the φ80lacZΔM15 insertion has the wild-type lacI -35 sequence, not the lacI<sup>q</sup> -35 sequence (gtgcaa) --[[User:Barry Canton\|BC]] 15:01, 29 March 2008 (EDT)
-	* leucine auxoroph (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/competent-cells-for-transformation/competent-cells-resources/genotypes-of-competent-cells.html [source])	+	* leucine auxoroph ([http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/competent-cells-for-transformation/competent-cells-resources/genotypes-of-competent-cells.html source])
	*References:		*References:
	**[http://cgsc2.biology.yale.edu/Strain.php?ID=11225 E. coli Genetic Stock Center, MC1061 Record]		**[http://cgsc2.biology.yale.edu/Strain.php?ID=11225 E. coli Genetic Stock Center, MC1061 Record]

Janet B. Matsen: /* TOP10 (Invitrogen) */

Janet B. Matsen — Wed, 13 Mar 2013 13:55:58 GMT

TOP10 (Invitrogen)

←Older revision		Revision as of 13:55, 13 March 2013
Line 515:		Line 515:
	** φ80lacZΔM15 actually contains the entire lac operon, including lacI<sup>q</sup> --[[User:Dcekiert\|Dcekiert]] 16:45, 23 January 2008 (CST)		** φ80lacZΔM15 actually contains the entire lac operon, including lacI<sup>q</sup> --[[User:Dcekiert\|Dcekiert]] 16:45, 23 January 2008 (CST)
	***Analysis of the published DH10B sequence (Genbank CP000948) suggests the φ80lacZΔM15 insertion has the wild-type lacI -35 sequence, not the lacI<sup>q</sup> -35 sequence (gtgcaa) --[[User:Barry Canton\|BC]] 15:01, 29 March 2008 (EDT)		***Analysis of the published DH10B sequence (Genbank CP000948) suggests the φ80lacZΔM15 insertion has the wild-type lacI -35 sequence, not the lacI<sup>q</sup> -35 sequence (gtgcaa) --[[User:Barry Canton\|BC]] 15:01, 29 March 2008 (EDT)
		+	* leucine auxoroph (http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/competent-cells-for-transformation/competent-cells-resources/genotypes-of-competent-cells.html [source])
	*References:		*References:
	**[http://cgsc2.biology.yale.edu/Strain.php?ID=11225 E. coli Genetic Stock Center, MC1061 Record]		**[http://cgsc2.biology.yale.edu/Strain.php?ID=11225 E. coli Genetic Stock Center, MC1061 Record]

Nagendra P Palani: /* Commonly used strains */

Nagendra P Palani — Tue, 05 Mar 2013 18:26:32 GMT

Commonly used strains

←Older revision		Revision as of 18:26, 5 March 2013
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	* See [http://cgsc2.biology.yale.edu/Strain.php?ID=9973 CGSC#6152] <br>		* See [http://cgsc2.biology.yale.edu/Strain.php?ID=9973 CGSC#6152] <br>
		+
		+	===MFDpir===
		+
		+	MG1655 RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecA
		+
		+	<i>E. coli </i> strain for performing biparental mating to transfer plasmids to other bacteria. This strain was constructed after noticing that the common conjugation strains, SM10 and S17-1, were Hfr+ and also transferring a Mu transposon to the recipient strain.
		+
		+	Refer >> Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of <i>Escherichia coli</i> Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery
		+
		+	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008518/

	===MG1655===		===MG1655===

Etienne Robillard: /* W3110 (λ857S7) */

Etienne Robillard — Mon, 04 Mar 2013 12:59:30 GMT

W3110 (λ857S7)

←Older revision		Revision as of 12:59, 4 March 2013
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	* New annotation has accession number DDBJ AP009048.		* New annotation has accession number DDBJ AP009048.
	===W3110 (λ857S7)===		===W3110 (λ857S7)===
-	~~F<sup>+</sup> λ<sup>-</sup> rph-1 INV(rrnD, rrnE)~~	+	* Phenotype: ?
-	* See ~~[[Endy:~~W3110(~~lambda857S7~~)]]	+	* See also the Talk page and the "W3110 (λ857S7)" section to discuss about this E. coli/K-12 strain.
-	* Check this~~...~~	+
-	** If the strain carry a prophage plasmid, then it is F+ and can mate with F- wildtype ''E.coli~~'' strains.~~ -~~[[User:Etienne Robillard\|Etienne Robillard]]~~ 12~~:37, 21 December 2012 (EST)~~	+

	===WM3064===		===WM3064===

Etienne Robillard: /* W3110 (λ857S7) */

Etienne Robillard — Fri, 21 Dec 2012 17:37:46 GMT

W3110 (λ857S7)

←Older revision		Revision as of 17:37, 21 December 2012
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	* New annotation has accession number DDBJ AP009048.		* New annotation has accession number DDBJ AP009048.
	===W3110 (λ857S7)===		===W3110 (λ857S7)===
-	F<sup>-</sup> λ<sup>-</sup> rph-1 INV(rrnD, rrnE)	+	F<sup>+</sup> λ<sup>-</sup> rph-1 INV(rrnD, rrnE)
	* See [[Endy:W3110(lambda857S7)]]		* See [[Endy:W3110(lambda857S7)]]
		+	* Check this...
		+	** If the strain carry a prophage plasmid, then it is F+ and can mate with F- wildtype ''E.coli'' strains. -[[User:Etienne Robillard\|Etienne Robillard]] 12:37, 21 December 2012 (EST)

	===WM3064===		===WM3064===

Etienne Robillard: /* W3110 */

Etienne Robillard — Fri, 21 Dec 2012 17:27:30 GMT

W3110

←Older revision		Revision as of 17:27, 21 December 2012
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	* See <cite>Horiuchi-MSB-2006</cite>. Briefly, there are 8 site (9nt) differences between W3110 and MG1655. They reside in 7 orgs and one rRNA gene. Two are nonfunctional (''rpoS'' and ''dcuA'') and 5 are unknown missense mutations.		* See <cite>Horiuchi-MSB-2006</cite>. Briefly, there are 8 site (9nt) differences between W3110 and MG1655. They reside in 7 orgs and one rRNA gene. Two are nonfunctional (''rpoS'' and ''dcuA'') and 5 are unknown missense mutations.
	* New annotation has accession number DDBJ AP009048.		* New annotation has accession number DDBJ AP009048.
		+	===W3110 (λ857S7)===
		+	F<sup>-</sup> λ<sup>-</sup> rph-1 INV(rrnD, rrnE)
		+	* See [[Endy:W3110(lambda857S7)]]

	===WM3064===		===WM3064===

Karmella Haynes: /* JM2.300 */

Karmella Haynes — Sun, 21 Oct 2012 17:59:11 GMT

JM2.300

←Older revision		Revision as of 17:59, 21 October 2012
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	*Some folks have been using this strain (i.e., Elowitz, Gardner) and it took me too long to find the CGSC#.		*Some folks have been using this strain (i.e., Elowitz, Gardner) and it took me too long to find the CGSC#.
	*This strain is no longer available from the CGSC		*This strain is no longer available from the CGSC
-	* This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R. Weiss*, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc. Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4.	+	* This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R. Weiss, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc. Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4.

	===LE392===		===LE392===

Karmella Haynes: /* JM2.300 */

Karmella Haynes — Sun, 21 Oct 2012 17:58:38 GMT

JM2.300

←Older revision		Revision as of 17:58, 21 October 2012
Line 326:		Line 326:
	*Some folks have been using this strain (i.e., Elowitz, Gardner) and it took me too long to find the CGSC#.		*Some folks have been using this strain (i.e., Elowitz, Gardner) and it took me too long to find the CGSC#.
	*This strain is no longer available from the CGSC		*This strain is no longer available from the CGSC
		+	* This strain may also be F-. JM2.300 is described as F-, lacI22, λ-, e14-, rpsL135(StrR), thi-1 in K. Brenner, D. Karig, R. Weiss*, F. Arnold, “Engineered biodirectional communication mediates a consensus in a microbial biofilm consortium,” in Proc. Natl. Acad. Sci. USA, 2007 Oct 30;104(44):17300-4.

	===LE392===		===LE392===

Janet B. Matsen: /* BL21(AI) */

Janet B. Matsen — Wed, 17 Oct 2012 22:21:39 GMT

BL21(AI)

←Older revision		Revision as of 22:21, 17 October 2012
Line 37:		Line 37:
	*an ''E. coli'' B strain carrying the T7 RNA polymerase gene in the ''araB'' locus of the ''araBAD'' operon<sup>q</sup>.		*an ''E. coli'' B strain carrying the T7 RNA polymerase gene in the ''araB'' locus of the ''araBAD'' operon<sup>q</sup>.
	*Transformed plasmids containing T7 promoter driven expression are repressed until L-arabinose induction of T7 RNA polymerase.		*Transformed plasmids containing T7 promoter driven expression are repressed until L-arabinose induction of T7 RNA polymerase.
		+	** Maximal expression is lower than that of BL21(DE3) (customer support 10/2012)
	*Derived from BL21.		*Derived from BL21.
	*See the [https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=595 product page] for more information.		*See the [https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=595 product page] for more information.