E. coli genotypes/Exhibit: Difference between revisions

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|-
|-
!Strain
!Strain
!Description
!Genotype
!Genotype
!Parent
!Parent
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|-
|-
|AG1
|AG1
|
|endA1;recA1;gyrA96;thi-1;relA1;glnV44;hsdR17(rK- mK+)
|endA1;recA1;gyrA96;thi-1;relA1;glnV44;hsdR17(rK- mK+)
|
|
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|-
|-
|BL21(DE3)
|BL21(DE3)
|
|F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5
|F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5
|B834
|B834
|
|
|
|
|protein expression
|T7 RNA polymerase
|prophage
|-
|BL21(DE3) pLysS",
|The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.
|F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR)
|BL21(DE3)
|
|chloramphenicol
|protein expression
|protein expression
|T7 RNA polymerase
|T7 RNA polymerase
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<script type="text/javascript">
<script type="text/javascript">
var data = {
var data = {
        {
            label : "BL21(DE3) pLysS",
            description : "The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.",
            purpose : "protein expression",
            resistance : "chloramphenicol",
            inducible : "T7 RNA polymerase",
            lambda : "prophage",
            parent : "BL21(DE3)",
            genotype : ["F-", "ompT", "gal", "dcm", "lon", "hsdSB(rB- mB-)", "lacIq", "lambda", "lacUV5-T7 gene 1", "ind1", "sam7", "nin5", "pLysS(cmR)"]
        },
         {
         {
             label : "BNN93",
             label : "BNN93",

Revision as of 14:37, 24 September 2007

This is a demo of Exhibit. It only includes some of the data from E. coli genotypes.

<exhibit> 

 <source id="ecoligenotypes" 
         columns="label,genotype,parent,requires,resistance,purpose,inducible,lambda" 
         type="Strain" label="Strain" pluralLabel="Strains" 
         />
 <facet facetClass="TextSearch" />
 <facet expression=".purpose" />
 <facet expression=".resistance" />
 <facet expression=".requires" />
 <facet expression=".genotype" />
 <view viewClass="Tabular"
       label="Table"
       columns=".label, .genotype, .parent, .purpose"
       sortColumn="0"
       />

</exhibit>

Strain Description Genotype Parent Requires Resistance Purpose Inducible Lambda
AG1 endA1;recA1;gyrA96;thi-1;relA1;glnV44;hsdR17(rK- mK+) thiamine nalidixic acid cloning
BL21(DE3) F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5 B834 protein expression T7 RNA polymerase prophage
BL21(DE3) pLysS", The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR) BL21(DE3) chloramphenicol protein expression T7 RNA polymerase prophage

<html> <script type="text/javascript"> var data = { 

       {
           label : "BNN93",
           lambda : "no",
           requires : ["thiamine", "threonine", "leucine"],
           genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "mcrB", "e14-(mcrA-)", "hsdR(rKmK)", "lambda-"]
       },
       {
           label : "BW26434 CGSC Strain #7658",
           lambda : "no",
           resistance : "kanamycin",
           genotype : ["D(araD-araB)567", "D(lacA-lacZ)514(::kan)", "lacIp-4000(lacIq)", "lambda-", "rpoS396(Am)?", "rph-1", "D(rhaD-rhaB)568", "hsdR514"]
       },
       {
           label : "C600",
           purpose : "general purpose",
           lambda : "no",
           requires : ["thiamine", "threonine", "leucine"],
           genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "lambda-"]
       },
       {
           label : "C600 hflA150 (Y1073, BNN102)",
           parent : "C600",
           purpose : "cDNA libraries",
           lambda : "no",
           resistance : "tetracycline",
           requires : ["thiamine", "threonine", "leucine"],
           genotype : ["F-", "thi-1", "thr-1", "leuB6", "lacY1", "tonA21", "glnV44", "lambda-", "hflA150(chr::Tn10)"]
       },
       {
           label : "D1210",
           parent : "HB101",
           genotype : ["lacIq", "lacY+"]
       },
       {
           label : "DB3.1",
           purpose : "propagating plasmids with ccdB",
           resistance : "streptomycin",
           parent : "HB101",
           genotype : ["F-", "gyrA462", "endA1", "glnV44", "D(sr1-recA)", "mcrB", "mrr", "hsdS20(rB-, mB-)", "ara14", "galK2", "lacY1", "proA2", "rpsL20(Smr)", "xyl5", "D(leu)", "mtl1"]
       },
       {
           label : "DH1",
           purpose : "cloning",
           resistance : "nalidixic acid",
           parent : "Hoffman-Berling 1100 strain derivative",
           genotype : ["endA1", "recA1", "gyrA96", "thi-1", "glnV44", "relA1", "hsdR17(rK- mK+)", "lambda-"]
       },
       {
           label : "DH5a",
           purpose : "cloning",
           resistance : "nalidixic acid",
           parent : "DH1",
           genotype : ["F-", "endA1", "glnV44", "thi-1", "recA1", "relA1", "gyrA96", "deoR", "nupG", "phi80dlacZD(M15)", "D(lacZYA-argF)U169", "hsdR17(rK- mK+)", "lambda-"]
       },
   ]

}; 

  </script>

</html>

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