E. coli genotypes/Exhibit
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{
label : "AG1",
purpose : "cloning",
resistance : "nalidixic acid",
requires : "thiamine",
genotype : ["endA1", "recA1", "gyrA96", "thi-1", "relA1", "glnV44", "hsdR17(rK- mK+)"]
},
{
label : "BL21(DE3)",
purpose : "protein expression",
inducible : "T7 RNA polymerase",
lambda : "prophage",
parent : "B834",
genotype : ["F-", "ompT", "gal", "dcm", "lon", "hsdSB(rB- mB-)", "lacIq", "lambda", "lacUV5-T7 gene 1", "ind1", "sam7", "nin5"]
},
{
label : "BL21(DE3) pLysS",
description : "The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.",
purpose : "protein expression",
resistance : "chloramphenicol",
inducible : "T7 RNA polymerase",
lambda : "prophage",
parent : "BL21(DE3)",
genotype : ["F-", "ompT", "gal", "dcm", "lon", "hsdSB(rB- mB-)", "lacIq", "lambda", "lacUV5-T7 gene 1", "ind1", "sam7", "nin5", "pLysS(cmR)"]
},
{
label : "BNN93",
lambda : "no",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "mcrB", "e14-(mcrA-)", "hsdR(rKmK)", "lambda-"]
},
{
label : "BW26434 CGSC Strain #7658",
lambda : "no",
resistance : "kanamycin",
genotype : ["D(araD-araB)567", "D(lacA-lacZ)514(::kan)", "lacIp-4000(lacIq)", "lambda-", "rpoS396(Am)?", "rph-1", "D(rhaD-rhaB)568", "hsdR514"]
},
{
label : "C600",
purpose : "general purpose",
lambda : "no",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "tonA21", "thi-1", "thr-1", "leuB6", "lacY1", "glnV44", "rfbC1", "fhuA1", "lambda-"]
},
{
label : "C600 hflA150 (Y1073, BNN102)",
parent : "C600",
purpose : "cDNA libraries",
lambda : "no",
resistance : "tetracycline",
requires : ["thiamine", "threonine", "leucine"],
genotype : ["F-", "thi-1", "thr-1", "leuB6", "lacY1", "tonA21", "glnV44", "lambda-", "hflA150(chr::Tn10)"]
},
{
label : "D1210",
parent : "HB101",
genotype : ["lacIq", "lacY+"]
},
{
label : "DB3.1",
purpose : "propagating plasmids with ccdB",
resistance : "streptomycin",
parent : "HB101",
genotype : ["F-", "gyrA462", "endA1", "glnV44", "D(sr1-recA)", "mcrB", "mrr", "hsdS20(rB-, mB-)", "ara14", "galK2", "lacY1", "proA2", "rpsL20(Smr)", "xyl5", "D(leu)", "mtl1"]
},
{
label : "DH1",
purpose : "cloning",
resistance : "nalidixic acid",
parent : "Hoffman-Berling 1100 strain derivative",
genotype : ["endA1", "recA1", "gyrA96", "thi-1", "glnV44", "relA1", "hsdR17(rK- mK+)", "lambda-"]
},
{
label : "DH5a",
purpose : "cloning",
resistance : "nalidixic acid",
parent : "DH1",
genotype : ["F-", "endA1", "glnV44", "thi-1", "recA1", "relA1", "gyrA96", "deoR", "nupG", "phi80dlacZD(M15)", "D(lacZYA-argF)U169", "hsdR17(rK- mK+)", "lambda-"]
},
]
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