E. coli genotypes/Exhibit
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<div id="exhibit-browse-panel" ex:facets=".purpose, .resistance, .requires, .lactose, .arabinose, .stopcodons, .Fplasmid, .lambda, .genotypetest"></div>
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<script type="text/javascript"> var data = {
items : [
{
label : "AG1",
purpose : "cloning",
resistance : "nalidixic acid",
requires : "thiamine",
stopcodons : "amber stop suppressed with glutamine",
genotypetest : ["endA1", "recA1", "gyrA96", "thi-1", "relA1", "glnV44", "hsdR17(rK- mK+)"]
},
{
label : "BL21(DE3)",
purpose : "protein expression",
Fplasmid : "F-",
lactose : "lacIq",
inducible : "T7 RNA polymerase",
lambda : "prophage",
parent : "B834",
genotype : "F- ompT gal dcm lon hsdSB(rB- mB-) lambda(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])"
},
{
label : "BL21(DE3) pLysS",
description : "The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced.",
purpose : "protein expression",
Fplasmid : "F-",
lactose : "lacIq",
resistance : "chloramphenicol",
inducible : "T7 RNA polymerase",
lambda : "prophage",
parent : "BL21(DE3)",
genotype : "F- ompT gal dcm lon hsdSB(rB- mB-) lambda(DE3) pLysS(cmR)"
},
{
label : "BNN93",
Fplasmid : "F-",
lambda : "no",
lactose : "lac permease deletion",
requires : "thiamine threonine leucine",
stopcodons : "amber stop suppressed with glutamine",
genotype : "F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 mcrB e14-(mcrA-) hsdR(rKmK) lambda-"
},
{
label : "BW26434 CGSC Strain #7658",
lambda : "no",
resistance : "kanamycin",
lactose : "operon deleted",
arabinose : "deletion",
genotype : "D(araD-araB)567, D(lacA-lacZ)514(::kan), lacIp-4000(lacIq), lambda-, rpoS396(Am)?, rph-1, D(rhaD-rhaB)568, hsdR514"
},
{
label : "C600",
Fplasmid : "F-",
purpose : "general purpose",
lambda : "no",
lactose : "lac permease deletion",
requires : "thiamine threonine leucine",
stopcodons : "amber stop suppressed with glutamine",
genotype : "F- tonA21 thi-1 thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1 lambda-"
},
{
label : "C600 hflA150 (Y1073, BNN102)",
parent : "C600",
Fplasmid : "F-",
purpose : "cDNA libraries",
lambda : "no",
resistance : "tetracycline",
lactose : "lac permease deletion",
requires : "thiamine threonine leucine",
stopcodons : "amber stop suppressed with glutamine",
genotype : "F- thi-1 thr-1 leuB6 lacY1 tonA21 glnV44 lambda- hflA150(chr::Tn10)"
},
]
};
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* You can optionally specify parameters:
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* Include your own styles here.
*/
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null // the type of item to show, if there are several types in your data
);
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* Code that you normally put in the onLoad handler for
* instantiating the exhibit and loading data.
*/
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includeScript(Exhibit_urlPrefix + "exhibit-api.js?bundle=false");
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