EDTA: Difference between revisions

Revision as of 21:46, 4 January 2006

Most enzymes that synthesize or modify nucleic acids (e.g. polymerases, ligases, kinases, nucleases) are Mg²⁺-dependent. The addition of EDTA is a convenient way to stop these reactions.

Here's a great page about EDTA, including formation constant (K_f) values for metal-EDTA complexes. Note that many heavy metal ions (like Fe³⁺, Co²⁺, and Zn²⁺) are chelated much more strongly than Mg²⁺. A little bit of EDTA in your reaction will go a long way to keep these evildoers out of trouble and away from your precious biomolecules without interfering with your Mg²⁺-dependent reactions.

Here's another article that speaks of the wonders of EDTA and how it can help treat Aunt Edna's cardiovascular disease, enhance your enjoyment of the Friday night happy hour, and act as a "get out of jail free" card.

@@ Line 1: / Line 1: @@
-*[http://www.chm.bris.ac.uk/motm/edta/edtah.htm Here's] a great page about EDTA, including [http://www.cem.msu.edu/~cem333/EDTATable.html formation constant] (K<sub>f</sub>) values for metal-EDTA complexes.
+*Most enzymes that synthesize or modify nucleic acids (e.g. polymerases, ligases, kinases, nucleases) are Mg<sup>2+</sup>-dependent. The addition of EDTA is a convenient way to stop these reactions.
+*[http://www.chm.bris.ac.uk/motm/edta/edtah.htm Here's] a great page about EDTA, including [http://www.cem.msu.edu/~cem333/EDTATable.html formation constant] (K<sub>f</sub>) values for metal-EDTA complexes. Note that many heavy metal ions (like Fe<sup>3+</sup>, Co<sup>2+</sup>, and Zn<sup>2+</sup>) are chelated much more strongly than Mg<sup>2+</sup>. A little bit of EDTA in your reaction will go a long way to keep these evildoers out of trouble and away from your precious biomolecules without interfering with your Mg<sup>2+</sup>-dependent reactions.
 *[http://www.vrp.com/art/963.asp Here's] another article that speaks of the wonders of EDTA and how it can help treat Aunt Edna's cardiovascular disease, enhance your enjoyment of the Friday night happy hour, and act as a "get out of jail free" card.