http://www.openwetware.org/index.php?title=EGTA&feed=atom&action=history 2013-06-20T10:18:57Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=EGTA&diff=315676&oldid=prev 2009-06-16T10:39:03Z

<p><span class="autocomment">EGTA 0.5M stock solution</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:39, 16 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O to 90ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O to 90ml</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0 with solid NaOH (<del class="diffchange diffchange-inline">~</del>4g)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0 with solid NaOH (<ins class="diffchange diffchange-inline">&gt;</ins>4g)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. <del class="diffchange diffchange-inline">The exactly </del>amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. <ins class="diffchange diffchange-inline">For pH 7.5 the exact </ins>amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Stability &amp; storage ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Stability &amp; storage ==</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315132&oldid=prev 2009-06-15T17:34:17Z

<p><span class="autocomment">EGTA 0.5M stock solution:&#32;</span> pH adjustment</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 17:34, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O to 90ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O to 90ml</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0 <ins class="diffchange diffchange-inline">with solid NaOH (~4g)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Note: EGTA will not go into solution without NaOH. Once the pH has been raised sufficiently it dissolves quickly. The exactly amount required is slightly above 4g. Add 3.5-4g immediately, then proceed carefully not to overshoot the desired pH.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Stability &amp; storage ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Stability &amp; storage ==</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315042&oldid=prev 2009-06-15T15:27:13Z

<p><span class="autocomment">Storage</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:27, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 12:</td> <td colspan="2" class="diff-lineno">Line 12:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Storage </del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Stability &amp; storage </ins>==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* room temperature</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* room temperature</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* for years</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* for years</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315040&oldid=prev 2009-06-15T15:26:39Z

<p>illustration</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:26, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:EGTA.png|thumb|right|300px|chemical structure of EDTA - ethylene-glycol-tetraacetic acid]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Ethylene glycol tetraacetic acid (EGTA)''' is a common buffer ingredient due to its chelating activity. It is similar to the better known [[EDTA]], but has a much higher affinity for calcium ions than for magnesium ions. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Ethylene glycol tetraacetic acid (EGTA)''' is a common buffer ingredient due to its chelating activity. It is similar to the better known [[EDTA]], but has a much higher affinity for calcium ions than for magnesium ions. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315028&oldid=prev 2009-06-15T15:01:54Z

<p>storage</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:01, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust pH 7.5/8.0</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* adjust volume to 100ml</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== Storage ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* room temperature</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* for years</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315011&oldid=prev 2009-06-15T14:07:47Z

<p>categories</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:07, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/EGTA_(chemical) Wikipedia: EGTA]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/EGTA_(chemical) Wikipedia: EGTA]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sigma catalogue EGTA [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E4378|SIAL&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E4378 100g 220€], [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E3889|SIGMA&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E3889 100g 253€] (as of 6/'09)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sigma catalogue EGTA [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E4378|SIAL&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E4378 100g 220€], [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E3889|SIGMA&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E3889 100g 253€] (as of 6/'09)</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Chemical]]</ins></div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315008&oldid=prev 2009-06-15T14:01:20Z

<p><span class="autocomment">EGTA 0.5M stock solution</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:01, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>100 ml solution</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>100 ml solution</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 19g EGTA (MW 380g/mol)</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* ddH2O</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* ddH2O <ins class="diffchange diffchange-inline">to 90ml</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* pH 7.5/8.0</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">adjust </ins>pH 7.5/8.0</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* adjust volume to 100ml</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== See also ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/EGTA_(chemical) Wikipedia: EGTA]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/EGTA_(chemical) Wikipedia: EGTA]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sigma catalogue EGTA [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E4378|SIAL&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E4378 100g 220€], [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E3889|SIGMA&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E3889 100g 253€] (as of 6/'09)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Sigma catalogue EGTA [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E4378|SIAL&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E4378 100g 220€], [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E3889|SIGMA&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E3889 100g 253€] (as of 6/'09)</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315004&oldid=prev 2009-06-15T13:52:50Z

<p><span class="autocomment">EGTA 0.5M stock solution</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:52, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== EGTA 0.5M stock solution ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== EGTA 0.5M stock solution ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>100 ml solution</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>100 ml solution</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">23.4g </del>EGTA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">19g </ins>EGTA <ins class="diffchange diffchange-inline">(MW 380g/mol)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* pH 7.5/8.0</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* pH 7.5/8.0</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=315000&oldid=prev 2009-06-15T13:49:53Z

<p>see also</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:49, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* ddH2O</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* pH 7.5/8.0</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* pH 7.5/8.0</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== See also ==</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://en.wikipedia.org/wiki/EGTA_(chemical) Wikipedia: EGTA]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Sigma catalogue EGTA [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E4378|SIAL&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E4378 100g 220€], [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=E3889|SIGMA&amp;N5=SEARCH_CONCAT_PNO|BRAND_KEY&amp;F=SPEC E3889 100g 253€] (as of 6/'09)</ins></div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale http://www.openwetware.org/index.php?title=EGTA&diff=314994&oldid=prev 2009-06-15T13:28:10Z

<p>lead section</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 13:28, 15 June 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''EGTA'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''<ins class="diffchange diffchange-inline">Ethylene glycol tetraacetic acid (</ins>EGTA<ins class="diffchange diffchange-inline">)</ins>''' <ins class="diffchange diffchange-inline">is a common buffer ingredient due to its chelating activity. It is similar to the better known [[EDTA]], but has a much higher affinity for calcium ions than for magnesium ions. Buffers made with EGTA are used in some cases to mimic the environment inside living cells where calcium ions are usually at least a thousandfold less concentrated than magnesium ions.</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>&#160;</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Full chemical name: ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== EGTA 0.5M stock solution ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== EGTA 0.5M stock solution ==</div></td></tr> <!-- diff generator: internal 2013-06-20 10:18:58 --> </table>

Jakob Suckale