Eccles: DGG Protocols: Difference between revisions
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= | ==Nucleic acid== | ||
*[[Eccles:RNA_extraction_AJ | Total RNA isolation with Tri Reagent and columns (Aaron)]] | |||
*[[Eccles:cDNA_Synthesis | cDNA synthesis (Jess Protocol)]] | |||
*[[Eccles:DNA Isolation | DNA isolation (Jody Protocol)]] | |||
*[[Eccles:PCR Product Sequencing | PCR product sequencing (Matt Protocol)]] | |||
*[[Eccles:qPCR_Reaction | qPCR reaction (Jess Protocol)]] | |||
*[[Eccles:random_panomer_hyb | Random oligo hyb (Aaron)]] | |||
*[[Ajeffs:bioanalyzer | Bioanalyzer (Aaron)]] | |||
* | ==Cell culture== | ||
* | *[[Eccles:cell_culture_CO2 | CO2 for cell culture]] | ||
* | *[[Eccles:siRNA_Transfection | siRNA transfection (Jess Protocol)]] | ||
* | *[[Eccles:AJ_siRNA_txn | siRNA forward transfection with Lipofectamine 2000 (Aaron)]] | ||
*[[Eccles:AJ_siRNA_rev_txn | siRNA reverse transfection with Lipofectamine 2000 (96-well) or RNAiMAX (24-well) (Aaron)]] | |||
*[[Eccles:Antisense_Transfection | Antisense transfection (Jess Protocol)]] | |||
*[[Eccles:NZM_Cell_Culture | Culturing NZM cell lines (Jess Protocol)]] | |||
*[[Eccles:Making_Primary_Cells_from_Tissue | Making primary cells from mouse tissue (Lana)]] | |||
*[[Eccles:Making_Primary_Cells_from_Surgery_Tissue | Making primary cells from surgery tissue (Lana)]] | |||
*[[Eccles:CB_Cell_Culture | Culturing CB cells (Mouse Embryonic Kidney Cells, SV40 large T positive) (Lana)]] | |||
*[[Eccles:Collagen_Coating_of_Cell_Culture_Vessels | Collagen coating of cell culture vessels (Lana)]] | |||
*[[Eccles:HuADPKD_Cell_Culture | Culturing human ADPKD primary kidney cells (Lana)]] | |||
*[[Eccles:MTT Assay (7d) on CB Cells | MTT Assay (7d) on CB Cells (Lana)]] | |||
*[[Eccles:MKS Epithelial Cell Culture (Lynn)]] | |||
==Protein== | |||
*[[Eccles:Western_Blotting | Western Blotting (Lana)]] | |||
*[[Eccles:Western_Blot_Stripping | Western Blot Stripping (Lana)]] | |||
*[[Eccles:Protein_Lysates_from_Cells_in_Culture | Protein lysates from cells in culture (Lana)]] | |||
*[[Eccles:Protein_Lysates_from_Tissue | Protein lysates from tissue (Lana)]] | |||
*[[Eccles:LS_MITF_westerns | MITF western protocol (Lynn)]] | |||
= | ==Genotyping== | ||
*[[Eccles:Pax2-1Neu_genotyping | Pax2-1Neu for WAVE dHPLC]] | |||
* | |||
Latest revision as of 18:58, 1 December 2009
Back to Eccles Lab
Nucleic acid
- Total RNA isolation with Tri Reagent and columns (Aaron)
- cDNA synthesis (Jess Protocol)
- DNA isolation (Jody Protocol)
- PCR product sequencing (Matt Protocol)
- qPCR reaction (Jess Protocol)
- Random oligo hyb (Aaron)
- Bioanalyzer (Aaron)
Cell culture
- CO2 for cell culture
- siRNA transfection (Jess Protocol)
- siRNA forward transfection with Lipofectamine 2000 (Aaron)
- siRNA reverse transfection with Lipofectamine 2000 (96-well) or RNAiMAX (24-well) (Aaron)
- Antisense transfection (Jess Protocol)
- Culturing NZM cell lines (Jess Protocol)
- Making primary cells from mouse tissue (Lana)
- Making primary cells from surgery tissue (Lana)
- Culturing CB cells (Mouse Embryonic Kidney Cells, SV40 large T positive) (Lana)
- Collagen coating of cell culture vessels (Lana)
- Culturing human ADPKD primary kidney cells (Lana)
- MTT Assay (7d) on CB Cells (Lana)
- Eccles:MKS Epithelial Cell Culture (Lynn)
Protein
- Western Blotting (Lana)
- Western Blot Stripping (Lana)
- Protein lysates from cells in culture (Lana)
- Protein lysates from tissue (Lana)
- MITF western protocol (Lynn)
