Eccles:genotyping oligos: Difference between revisions
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===KRD PRIMERS=== | ===KRD PRIMERS=== | ||
{{hide|1= | |||
*Organism: Mus Musculus | *Organism: Mus Musculus | ||
*Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp) | *Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp) | ||
| Line 21: | Line 19: | ||
*approx 250 bp | *approx 250 bp | ||
*Amplifies a portion of the CAT transgene | *Amplifies a portion of the CAT transgene | ||
}} | |||
===GFP PRIMERS=== | ===GFP PRIMERS=== | ||
{{hide|1= | |||
*Organism: Mus Musculus | *Organism: Mus Musculus | ||
*Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp) | *Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp) | ||
| Line 40: | Line 36: | ||
*Forward primer in the eGFP cDNA and the reverse is in the globin sequence | *Forward primer in the eGFP cDNA and the reverse is in the globin sequence | ||
*Programme - Jody - GFP on red machine | *Programme - Jody - GFP on red machine | ||
}} | |||
===MEST PRIMERS=== | ===MEST PRIMERS=== | ||
{{hide|1= | |||
*Organism: Mus Musculus | *Organism: Mus Musculus | ||
*Forward Primer: AAGACGGAGGTGTGCTTTCC (20 bp) | *Forward Primer: AAGACGGAGGTGTGCTTTCC (20 bp) | ||
| Line 60: | Line 54: | ||
**Current [stock]: | **Current [stock]: | ||
*Annealing Temperature: 58 °C, 1 minute extension | *Annealing Temperature: 58 °C, 1 minute extension | ||
*8F and 9R bind to exon 8 and 9 of the | *8F and 9R bind to exon 8 and 9 of the wildt ype Mest gene and give a PCR product of 314 bp (wildtype). The mutant band is larger. | ||
*Programme, on Red machine - Jody - Mest | *Programme, on Red machine - Jody - Mest | ||
}} | |||
===Pax2-1Neu=== | |||
{{hide|1= | |||
*Forward Primer: gggcacgggggtgtgaaccag | |||
**DGG ID: 2292 | |||
**DGG name: P2PB1F | |||
**Current [stock]: | |||
*Reverse Primer: ctgcccaggattttgctgacacagcc | |||
**DGG ID: 2293 | |||
**DGG name: PAX2EXTR | |||
**Current [stock]: | |||
*Amplicons: 167 (wt), 168 (mutant) | |||
}} | |||
Latest revision as of 15:30, 11 October 2007
Eccles Lab > DGG Oligos > genotyping_oligos >
MOUSE
KRD PRIMERS
- Organism: Mus Musculus
- Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp)
- DGG ID:
- DGG name: CATF
- Current [stock]:
- Reverse Primer: CCAGACCGTTCAGCTGGATATTACGGCCTT (30 bp)
- DGG ID:
- DGG name: CATR
- Current [stock]:
- Annealing Temperature: 72 °C
- approx 250 bp
- Amplifies a portion of the CAT transgene
GFP PRIMERS
- Organism: Mus Musculus
- Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp)
- DGG ID:
- DGG name: GFPF
- Current [stock]:
- Reverse Primer: ACG ATC CTG AGA CTT CCA CAC T (22 bp)
- DGG ID:
- DGG name: GFPR
- Current [stock]:
- Annealing Temperature: 54°C
- 321 bp
- Forward primer in the eGFP cDNA and the reverse is in the globin sequence
- Programme - Jody - GFP on red machine
MEST PRIMERS
- Organism: Mus Musculus
- Forward Primer: AAGACGGAGGTGTGCTTTCC (20 bp)
- DGG ID:
- DGG name: Mest8F
- Current [stock]:
- Common Primer: TGTCGATGACCAGGTTGCC (19 bp)
- DGG ID:
- DGG name: MEST9R
- Current [stock]:
- Reverse Primer: GCATCGCCTTCTATCGCCTT (20 bp)
- DGG ID:
- DGG name: MEST10FJ
- Current [stock]:
- Annealing Temperature: 58 °C, 1 minute extension
- 8F and 9R bind to exon 8 and 9 of the wildt ype Mest gene and give a PCR product of 314 bp (wildtype). The mutant band is larger.
- Programme, on Red machine - Jody - Mest
Pax2-1Neu
- Forward Primer: gggcacgggggtgtgaaccag
- DGG ID: 2292
- DGG name: P2PB1F
- Current [stock]:
- Reverse Primer: ctgcccaggattttgctgacacagcc
- DGG ID: 2293
- DGG name: PAX2EXTR
- Current [stock]:
- Amplicons: 167 (wt), 168 (mutant)
