Eccles:genotyping oligos: Difference between revisions
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**Current [stock]: | **Current [stock]: | ||
*Annealing Temperature: 58 °C, 1 minute extension | *Annealing Temperature: 58 °C, 1 minute extension | ||
*8F and 9R bind to exon 8 and 9 of the | *8F and 9R bind to exon 8 and 9 of the wildt ype Mest gene and give a PCR product of 314 bp (wildtype). The mutant band is larger. | ||
*Programme, on Red machine - Jody - Mest | *Programme, on Red machine - Jody - Mest | ||
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===Pax2-1Neu=== | ===Pax2-1Neu=== | ||
{{hide|1= | {{hide|1= | ||
*Forward Primer: | *Forward Primer: gggcacgggggtgtgaaccag | ||
**DGG ID: 2292 | **DGG ID: 2292 | ||
**DGG name: P2PB1F | **DGG name: P2PB1F | ||
**Current [stock]: | **Current [stock]: | ||
*Reverse Primer: | *Reverse Primer: ctgcccaggattttgctgacacagcc | ||
**DGG ID: 2293 | **DGG ID: 2293 | ||
**DGG name: PAX2EXTR | **DGG name: PAX2EXTR | ||
**Current [stock]: | **Current [stock]: | ||
*Amplicons: 167 (wt), 168 (mutant) | |||
}} | }} |
Latest revision as of 15:30, 11 October 2007
Eccles Lab > DGG Oligos > genotyping_oligos >
MOUSE
KRD PRIMERS
- Organism: Mus Musculus
- Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp)
- DGG ID:
- DGG name: CATF
- Current [stock]:
- Reverse Primer: CCAGACCGTTCAGCTGGATATTACGGCCTT (30 bp)
- DGG ID:
- DGG name: CATR
- Current [stock]:
- Annealing Temperature: 72 °C
- approx 250 bp
- Amplifies a portion of the CAT transgene
GFP PRIMERS
- Organism: Mus Musculus
- Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp)
- DGG ID:
- DGG name: GFPF
- Current [stock]:
- Reverse Primer: ACG ATC CTG AGA CTT CCA CAC T (22 bp)
- DGG ID:
- DGG name: GFPR
- Current [stock]:
- Annealing Temperature: 54°C
- 321 bp
- Forward primer in the eGFP cDNA and the reverse is in the globin sequence
- Programme - Jody - GFP on red machine
MEST PRIMERS
- Organism: Mus Musculus
- Forward Primer: AAGACGGAGGTGTGCTTTCC (20 bp)
- DGG ID:
- DGG name: Mest8F
- Current [stock]:
- Common Primer: TGTCGATGACCAGGTTGCC (19 bp)
- DGG ID:
- DGG name: MEST9R
- Current [stock]:
- Reverse Primer: GCATCGCCTTCTATCGCCTT (20 bp)
- DGG ID:
- DGG name: MEST10FJ
- Current [stock]:
- Annealing Temperature: 58 °C, 1 minute extension
- 8F and 9R bind to exon 8 and 9 of the wildt ype Mest gene and give a PCR product of 314 bp (wildtype). The mutant band is larger.
- Programme, on Red machine - Jody - Mest
Pax2-1Neu
- Forward Primer: gggcacgggggtgtgaaccag
- DGG ID: 2292
- DGG name: P2PB1F
- Current [stock]:
- Reverse Primer: ctgcccaggattttgctgacacagcc
- DGG ID: 2293
- DGG name: PAX2EXTR
- Current [stock]:
- Amplicons: 167 (wt), 168 (mutant)