Eccles:genotyping oligos

Eccles Lab > DGG Oligos > genotyping_oligos >

MOUSE

KRD PRIMERS

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• Organism: Mus Musculus
• Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp)
  • DGG ID:
  • DGG name: CATF
  • Current [stock]:
• Reverse Primer: CCAGACCGTTCAGCTGGATATTACGGCCTT (30 bp)
  • DGG ID:
  • DGG name: CATR
  • Current [stock]:
• Annealing Temperature: 72 °C
• approx 250 bp
• Amplifies a portion of the CAT transgene

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GFP PRIMERS

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• Organism: Mus Musculus
• Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp)
  • DGG ID:
  • DGG name: GFPF
  • Current [stock]:
• Reverse Primer: ACG ATC CTG AGA CTT CCA CAC T (22 bp)
  • DGG ID:
  • DGG name: GFPR
  • Current [stock]:
• Annealing Temperature: 54°C
• 321 bp
• Forward primer in the eGFP cDNA and the reverse is in the globin sequence
• Programme - Jody - GFP on red machine

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