EcoRI

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(References)
(References)
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// No abstract available online.
// No abstract available online.
#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
#Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
 +
// Use of EcoRI to generate DNA fragments that can be ligated
 +
#Cohen-PNAS-1972 pmid=4594039
// Use of EcoRI to generate recombinant DNA fragments
// Use of EcoRI to generate recombinant DNA fragments
</biblio>
</biblio>
[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]
[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Revision as of 15:51, 20 December 2007

Contents

Properties

Recognition site

EcoR-I-cutsite_1.gif

Buffers

NEBuffer EcoR I

Notes

  • Improving the efficiency of EcoRI/SpeI Double Digest.
  • Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
  • The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.

External links

EcoRI from NEB
EcoRI from Promega

References

Error fetching PMID 4343967:
Error fetching PMID 4343959:
Error fetching PMID 4343974:
Error fetching PMID 4578426:
Error fetching PMID 4343968:
Error fetching PMID 4594039:
  1. Error fetching PMID 4343967: [Morrow-PNAS-1972]
    Discusses cleavage of DNA at a unique location by EcoRI

  2. Error fetching PMID 4343959: [Mulder-PNAS-1972]
    Specificity of EcoRI

  3. Error fetching PMID 4343974: [Hedgpeth-Proc-Natl-Acad-Sci-USA-1972]
    Identifies the overhang sequence produced by EcoRI

  4. Error fetching PMID 4578426: [Bigger-Nat-New-Biol-1973]
    No abstract available online.

  5. Error fetching PMID 4343968: [Mertz-Proc-Natl-Acad-Sci-USA-1972]
    Use of EcoRI to generate DNA fragments that can be ligated

  6. Error fetching PMID 4594039: [Cohen-PNAS-1972]
    Use of EcoRI to generate recombinant DNA fragments

All Medline abstracts: PubMed HubMed
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