EcoRI
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| + | ==Properties== | ||
| + | ===Recognition site=== | ||
| + | http://www.neb.com/nebecomm/productfiles/314/images/EcoR-I-cutsite_1.gif | ||
| + | |||
| + | ===Buffers=== | ||
| + | NEBuffer EcoR I | ||
| + | |||
| + | ==Notes== | ||
*Improving the efficiency of [[EcoRI/SpeI Double Digest]]. | *Improving the efficiency of [[EcoRI/SpeI Double Digest]]. | ||
*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) | *'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) | ||
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| + | ==References== | ||
| + | [http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB] | ||
Revision as of 10:57, 3 May 2006
Contents |
Properties
Recognition site
Buffers
NEBuffer EcoR I
Notes
- Improving the efficiency of EcoRI/SpeI Double Digest.
- Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
