EcoRI: Difference between revisions
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==Properties== | |||
===Recognition site=== | |||
http://www.neb.com/nebecomm/productfiles/314/images/EcoR-I-cutsite_1.gif | |||
===Buffers=== | |||
NEBuffer EcoR I | |||
==Notes== | |||
*Improving the efficiency of [[EcoRI/SpeI Double Digest]]. | *Improving the efficiency of [[EcoRI/SpeI Double Digest]]. | ||
*'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) | *'''Reaction Volumes''' - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --[[User:Bcanton|BC]] 13:39, 2 Jun 2005 (EDT) | ||
==References== | |||
[http://www.neb.com/nebecomm/products/productR0101.asp EcoRI from NEB] | |||
Revision as of 07:57, 3 May 2006
Properties
Recognition site
http://www.neb.com/nebecomm/productfiles/314/images/EcoR-I-cutsite_1.gif
Buffers
NEBuffer EcoR I
Notes
- Improving the efficiency of EcoRI/SpeI Double Digest.
- Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
