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Recognition site



NEBuffer EcoR I


  • Improving the efficiency of EcoRI/SpeI Double Digest.
  • Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
  • The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.
  • The FastDigest restriction enzymes from Fermentas all work very efficiently in one universal buffer. I perform digests in a 30 μL reaction volume. *Karmella Haynes 14:07, 19 January 2012 (EST):

External links

EcoRI from NEB
EcoRI from Promega


Error fetching PMID 4343967:
Error fetching PMID 4343959:
Error fetching PMID 4343974:
Error fetching PMID 4578426:
Error fetching PMID 4343968:
Error fetching PMID 4594039:
  1. Error fetching PMID 4343967: [Morrow-PNAS-1972]
    Discusses cleavage of DNA at a unique location by EcoRI

  2. Error fetching PMID 4343959: [Mulder-PNAS-1972]
    Specificity of EcoRI

  3. Error fetching PMID 4343974: [Hedgpeth-Proc-Natl-Acad-Sci-USA-1972]
    Identifies the overhang sequence produced by EcoRI

  4. Error fetching PMID 4578426: [Bigger-Nat-New-Biol-1973]
    No abstract available online.

  5. Error fetching PMID 4343968: [Mertz-Proc-Natl-Acad-Sci-USA-1972]
    Use of EcoRI to generate DNA fragments that can be ligated

  6. Error fetching PMID 4594039: [Cohen-PNAS-1973]
    Use of EcoRI to generate recombinant DNA fragments

All Medline abstracts: PubMed HubMed
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