Elizabeth Polidan Week11: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Forgot template -- added it.) |
(Adding vocabulary) |
||
| Line 3: | Line 3: | ||
#trehalose | #trehalose | ||
#*A nonreducing disaccharide that acts as a storage carbohydrate and protects cells against various environmental stress conditions. | |||
#*''Charlemagne-Gilles, H., et al., Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress, Microbiology (1998), 144, pp. 671-680. (http://mic.sgmjournals.org/content/144/3/671.full.pdf)'' | |||
#mannoproteins | #mannoproteins | ||
#*Yeast cell wall components composed of proteins with mannose groups attached. | |||
#*''http://www.biology-online.org/dictionary/Mannoproteins'' | |||
#prototrophic | #prototrophic | ||
#*A strain that has the same nutritional requirements as the wild strain from which it is derived. | |||
#*''http://www.biology-online.org/dictionary/Prototrophic_strains'' | |||
#fatty-acid desaturase | #fatty-acid desaturase | ||
#*Enzymes that put double bonds into the hydrocarbon areas of fatty acids. | |||
#*''http://www.biology-online.org/dictionary/Desaturase'' | |||
#cis-regulatory motifs | #cis-regulatory motifs | ||
#*A region of DNA or RNA that regulates the expression of genes also located on that same strand of DNA or RNA. | |||
#*''http://en.wikipedia.org/wiki/Cis-regulatory_element'' | |||
#HXT5 | #HXT5 | ||
#*Hexose transporter with moderate affinity for glucose. | |||
#*''http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=HXT5'' | |||
#HXT6 | #HXT6 | ||
#*High-affinity glucose transporter | |||
#*''http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=hxt6'' | |||
#Carbon recovery | |||
#*Ratio of amount of recovered carbon to the amount available at the onset of fermentation. It is a measure of efficiency. Usually percentage reflecting # moles produced per 100 moles of substrate used. | |||
#*''El-Mansi, E.M.T, Bryce, C.F.A., Demain, A.L., and Allman, A.R. editors (2006) Fermentation Microbiology and Biothechnology, 2nd edition, Boca Raton, FL: CRC Press, p. 31.'' | |||
'''Outline of Tai et al. (2007)''' | '''Outline of Tai et al. (2007)''' | ||
Revision as of 19:26, 3 April 2013
Elizabeth Polidan
BIOL 398.03 / MATH 388
- Loyola Marymount University
- Los Angeles, CA, USA
Vocabulary
- trehalose
- A nonreducing disaccharide that acts as a storage carbohydrate and protects cells against various environmental stress conditions.
- Charlemagne-Gilles, H., et al., Role of trehalose in survival of Saccharomyces cerevisiae under osmotic stress, Microbiology (1998), 144, pp. 671-680. (http://mic.sgmjournals.org/content/144/3/671.full.pdf)
- mannoproteins
- Yeast cell wall components composed of proteins with mannose groups attached.
- http://www.biology-online.org/dictionary/Mannoproteins
- prototrophic
- A strain that has the same nutritional requirements as the wild strain from which it is derived.
- http://www.biology-online.org/dictionary/Prototrophic_strains
- fatty-acid desaturase
- Enzymes that put double bonds into the hydrocarbon areas of fatty acids.
- http://www.biology-online.org/dictionary/Desaturase
- cis-regulatory motifs
- A region of DNA or RNA that regulates the expression of genes also located on that same strand of DNA or RNA.
- http://en.wikipedia.org/wiki/Cis-regulatory_element
- HXT5
- Hexose transporter with moderate affinity for glucose.
- http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=HXT5
- HXT6
- High-affinity glucose transporter
- http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=hxt6
- Carbon recovery
- Ratio of amount of recovered carbon to the amount available at the onset of fermentation. It is a measure of efficiency. Usually percentage reflecting # moles produced per 100 moles of substrate used.
- El-Mansi, E.M.T, Bryce, C.F.A., Demain, A.L., and Allman, A.R. editors (2006) Fermentation Microbiology and Biothechnology, 2nd edition, Boca Raton, FL: CRC Press, p. 31.
Outline of Tai et al. (2007)
- Introduction
- What is the main result presented in this paper?
- What is the importance or significance of this work?
- Methods
- How did they treat the cells (what experiment were they doing?)
- What strain(s) of yeast did they use? Was the strain haploid or diploid?
- What media did they grow them in? Under what conditions and temperatures?
- What controls did they use?
- How many replicates did they perform per condition?
- Analysis
- What mathematical/statistical method did they use to analyze the data?
- Discussion
- What transcription factors did they talk about?
- Briefly state the result shown in each of the figures and tables.
Placeholder for reflection questions.
Critically evaluate the Tai et al. (2007) paper.
- Overall, do you think this paper was clearly written? Why or why not?
- Based on what is written in the methods section, do you think you could reproduce their experiments and data analysis?
- What else would you like to know about their methods, results, and future directions?
