Endy:Autoclaving biohazard waste: Difference between revisions
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#Put the bin in the autoclave and run the autoclave on the 'fast' cycle for 1hr at 250F | #Put the bin in the autoclave and run the autoclave on the 'fast' cycle for 1hr at 250F | ||
#Wait for cycle to complete and pressure to decrease | #Wait for cycle to complete and pressure to decrease | ||
#Open autoclave ( | #Open autoclave (remember to open the door slowly to allow steam to escape and be careful not to bumping against the very hot door), and remove the bin. | ||
#Pull the test vial out of the autoclave waste using the string. | #Pull the test vial out of the autoclave waste using the string. | ||
#*This should be returned to the 2nd floor room and a label affixed indicating the date, investigator, etc (labels are next to the drop off box). | #*This should be returned to the 2nd floor room and a label affixed indicating the date, investigator, etc (labels are next to the drop off box or by Heather's desk). | ||
#Seal off the waste using the metal twist-tie attached to the waste tag and then dospose of in the regular trash. | #Seal off the waste using the metal twist-tie attached to the waste tag and then dospose of in the regular trash. |
Revision as of 13:20, 19 June 2006
This page describes the process for autoclaving waste in the biology building at MIT.
Materials
1 bag of waste (double bagged in clear biohazard bags)
Steps
- Get one of the test vials from 2nd floor (in same room as 5th floor media room, what room#?) and a piece of string about 3 ft long.
- Wrap the string aroung the vial, knot it, and secure it with tape
- Lower the test vial into the center of the autoclave waste (or as close as you can get) leaving the string hanging outside of the bin.
- Put the bag into an autoclave-safe bin and bring it to the autoclave
- Note: the bag should be as open as possible without the contents spilling out, in order to enable the steam to better penetrate the waste
- Attach the 'Autoclaved Non-infectious Biomedical Waste' tag to the bag
- Note: try and have this hanging outside the bin so that if their is a liquid pool after the autoclave run, the tag wont be damaged.
- Put the bin in the autoclave and run the autoclave on the 'fast' cycle for 1hr at 250F
- Wait for cycle to complete and pressure to decrease
- Open autoclave (remember to open the door slowly to allow steam to escape and be careful not to bumping against the very hot door), and remove the bin.
- Pull the test vial out of the autoclave waste using the string.
- This should be returned to the 2nd floor room and a label affixed indicating the date, investigator, etc (labels are next to the drop off box or by Heather's desk).
- Seal off the waste using the metal twist-tie attached to the waste tag and then dospose of in the regular trash.