Endy:Back Door: Difference between revisions

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==Stanford==
==Stanford==


===New to Lab?===
===New to the lab?===
*Start [[Endy:Starting_Out | here]]
*Start [[Endy:Starting_Out | here]] (includes safety training, '''you really must start here''')


===Moving On?===
===Moving on?===
*End [[Endy:Winding_Up | here]]
*End [[Endy:Winding_Up | here]]
===Lab Evacuation & Rally Points===
*Evacuation plans [[Endy:Evacuation | here]]


===Group Meeting===
===Group Meeting===
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** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time)
** [[Endy:Making 1% Agarose stock | 1% agarose]] ('''TBD''' depends how we want to proceed e.g. common/personal stock or make it each time)
** Sharps ('''Monica''')
** Sharps ('''Monica''')
** Librarian (Books / Catalogs / Software / Manuals) ('''Cesar''')
** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] ('''Jerome''')
** [[Endy:Preparing Antibiotic Stocks|Preparing Antibiotic Stocks]] ('''Francois''')
** BB enzymes & ligase ('''Monica''')
** [[Qiagen Kits | Qiagen or other kits]] ('''Jerome''')
** Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) ('''Paul''')
** Enzymes ('''Jerome''')
** Strains ('''Jerome''' make an inventory and a stock)
** Strains ('''Jerome & Francois ?''' make an inventory and a stock)
** TAE / TBE / MilliQ ('''Ton''')
** TAE / TBE / MilliQ ('''Jerome''')
** Pouring [[Agar plates]] ('''Monica, Jerome, and Ton''')
** Pouring Agar plates ('''TBD''' probably a rotation could be good)
** Preparing competent cells [DH5 alpha Z1] ('''Jerome''' and '''Ton''')
** Preparing TOP10 competent cells
** Hazardous waste removal (no real need yet)
** Hazardous waste removal (no real need yet)
** Glassware ('''Elizabeth''')
** Glassware ('''Julie''')
** Ordering / Inventory of Supplies  ('''Jean/Elizabeth''')
** Ordering / Inventory of Supplies  ('''Jean/Julie''')
** Pipette Tip Box Recycling ('''Elizabeth''')
** Pipette Tip Box Recycling [1x per month, put empty boxes in container] ('''Julie''')
** Autoclaving culture plates ('''Elizabeth''')
** Autoclaving culture plates ('''Julie''')
**[[Washroom_User_Protocols|Washroom User Protocols]] ('''Elizabeth''')
**[[Washroom_User_Protocols|Washroom User Protocols]] ('''Julie''')


* EQUIPMENT
* EQUIPMENT
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** Centrifuges ('''Ton''')
** Centrifuges ('''Ton''')
** Hot plates / dri-baths / waterbaths ('''Ton''')
** Hot plates / dri-baths / waterbaths ('''Ton''')
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room ('''Francois & Monica?''')
** -20C Freezers, [[Endy:-80C freezers | -80C freezers]], fridges, cold room
** System backup ('''Cesar''')
*** Defrosting enzyme blocks ('''Monica''')
** [[Endy:Printers|Lab Printers]] ('''Cesar''')
*** Defrosting -20C freezers ('''All''')
** MilliQ station? ('''Jerome''')


* SPACES:
* SPACES:
** Shared Space with Smolke Lab
** Shared Space with Smolke Lab
** [[Endy:Gel area | Gel Area]] ('''Elizabeth''')
** [[Endy:Gel area | Gel Area]] ('''Julie''')


*GETTING STARTED IN THE LAB
*GETTING STARTED IN THE LAB
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**[[Endy:Winding_Up|Check List for the Graceful Exit]]
**[[Endy:Winding_Up|Check List for the Graceful Exit]]


===Vacation Coverage for EAV===
===Vacation Coverage===
*[[Vacation_coverage|Sign-ups for vacation coverage]]
*[[Vacation_coverage|Sign-ups for vacation coverage]]
*[[Smolke:Dec shutdown|Shutdown]]


===Journal Club===
===Journal Club===
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[[MIT Era|Archives]]
[[MIT Era|Archives]]
[[Image:OWW Logo.png|150px |link=http://www.example.org |alt=Alt text|Title text]]

Revision as of 11:10, 12 March 2013

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Stanford

New to the lab?

  • Start here (includes safety training, you really must start here)

Moving on?

Lab Evacuation & Rally Points

Group Meeting

Lab Meetings - Time, location, and schedule listed here!

Making group meetings more effective

Lab Life Responsibilities

  • TASKS:
    • Lab Meetings: room reservation, scheduling of speakers, managing the clock (Ton)
    • 1% agarose (TBD depends how we want to proceed e.g. common/personal stock or make it each time)
    • Sharps (Monica)
    • Preparing Antibiotic Stocks (Jerome)
    • BB enzymes & ligase (Monica)
    • Polymerases (Hi-Fi supermix, Kappa, Pfu Ultra II) (Paul)
    • Strains (Jerome make an inventory and a stock)
    • TAE / TBE / MilliQ (Ton)
    • Pouring Agar plates (Monica, Jerome, and Ton)
    • Preparing competent cells [DH5 alpha Z1] (Jerome and Ton)
    • Hazardous waste removal (no real need yet)
    • Glassware (Julie)
    • Ordering / Inventory of Supplies (Jean/Julie)
    • Pipette Tip Box Recycling [1x per month, put empty boxes in container] (Julie)
    • Autoclaving culture plates (Julie)
    • Washroom User Protocols (Julie)
  • EQUIPMENT
    • Microscopes (Francois)
    • Plate reader (Monica)
    • Centrifuges (Ton)
    • Hot plates / dri-baths / waterbaths (Ton)
    • -20C Freezers, -80C freezers, fridges, cold room
      • Defrosting enzyme blocks (Monica)
      • Defrosting -20C freezers (All)
  • SPACES:
    • Shared Space with Smolke Lab
    • Gel Area (Julie)

Vacation Coverage

Journal Club

SynBERC 2009

MIT ERA

Archives

Alt text

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