Endy:CC4249: Difference between revisions
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* '''Stock preparation:''' The stock came as a transport swab. The swab was dipped in 5mL of LB and grown at 37C. After ~1-2hours of growth, ampicillin (20ug/ml) and tetracycline (10ug/ml) were added. Growth was stopped when the culture reached OD600~2.9 (saturation). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C. | * '''Stock preparation:''' The stock came as a transport swab. The swab was dipped in 5mL of LB and grown at 37C. After ~1-2hours of growth, ampicillin (20ug/ml) and tetracycline (10ug/ml) were added. Growth was stopped when the culture reached OD600~2.9 (saturation). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C. | ||
* '''Physical location of the stock:''' | * '''Physical location of the stock:''' | ||
** [[User:Stpierre|Francois St-Pierre]]'s stocks: 2 vials in box "Francois's Ecoli 1" (as of Oct3,2005) | ** [[User:Stpierre|Francois St-Pierre]]'s stocks: 2 vials in box "Francois's Ecoli 1" (as of Oct3,2005). ID#: FSP B6. | ||
Revision as of 11:55, 3 October 2005
Back to Endy lab E coli strains
- Strain: CC4249
- Date: 09/2005
- Experimenter: Francois St-Pierre
- Strain obtained from: Jim Sawitzke, Austin Lab (NIH)
- Stock preparation: The stock came as a transport swab. The swab was dipped in 5mL of LB and grown at 37C. After ~1-2hours of growth, ampicillin (20ug/ml) and tetracycline (10ug/ml) were added. Growth was stopped when the culture reached OD600~2.9 (saturation). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
- Physical location of the stock:
- Francois St-Pierre's stocks: 2 vials in box "Francois's Ecoli 1" (as of Oct3,2005). ID#: FSP B6.
