Endy:Chassis engineering/VM2.0: Difference between revisions

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What are the metrics for each of the design considerations?
What are the metrics for each of the design considerations?
<br style="clear:both" />
<br style="clear:both" />
==Species==
#T7 RNAP
#Repressor
#Ribosomes<font color="green">
#Repressible T7 promoter
#T7RNAP-promoter complex
#Repressor-promoter complex</font><font color="red">
#T7 RNAP mRNA
#Repressor mRNA
#Elongating T7 RNAP
#Elongating Ribosomes
#etc.</font>

Revision as of 14:47, 7 March 2007

VM2.0 design considerations

Regulation scheme for VM2.0
  1. Stability
    • Noise
    • Response time
  2. Self-booting/controlled
    • Ability to turn on or off
  3. Tunable
    • Pros and cons of DNA copy number, promoter strength, repressor affinities etc.
  4. Efficient
    • Minimizing levels of repressor needed
    • Minimizing consumption of small molecules

What are the metrics for each of the design considerations?

Species

  1. T7 RNAP
  2. Repressor
  3. Ribosomes
  4. Repressible T7 promoter
  5. T7RNAP-promoter complex
  6. Repressor-promoter complex
  7. T7 RNAP mRNA
  8. Repressor mRNA
  9. Elongating T7 RNAP
  10. Elongating Ribosomes
  11. etc.
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