Endy:Chassis engineering/VM2.0

VM2.0 design considerations

Regulation scheme for VM2.0

1. Stability
   • Noise
   • Response time
2. Self-booting/controlled
   • Ability to turn on or off
3. Tunable
   • Pros and cons of DNA copy number, promoter strength, repressor affinities etc.
4. Efficient
   • Minimizing levels of repressor needed
   • Minimizing consumption of small molecules

What are the metrics for each of the design considerations?

Species

1. T7 RNAP
2. Repressor
3. Ribosomes
4. Repressible T7 promoter
5. T7RNAP-promoter complex
6. Repressor-promoter complex
7. T7 RNAP mRNA
8. Repressor mRNA
9. Elongating T7 RNAP
10. Elongating Ribosomes
11. etc.