Endy:Chemostat

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(Setup)
(Masterflex tubing in the pump)
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===Temperature consistency in water bath===
===Temperature consistency in water bath===
===Masterflex tubing in the pump===
===Masterflex tubing in the pump===
-
How did we avoid crushing it?
+
avoid crushing this tubing by removing the clamp on the parastaltic pump when it is not running.
 +
 
===Contamination===
===Contamination===
Others?
Others?

Revision as of 18:38, 17 June 2006

This page is for storing information about operation of a bench-scale chemostat designed by Ben Kirkup. It is in development.

Contents

Design

  • <photo>
  • <CAD design> (ask ben if OK)

Setup

  1. Wash out chemostat. don't lose the little glass beads.
  2. autoclave making sure that all tubes are connected.
  3. setup everything and run media through the device for a little while. this is a good time to calculate the flow rate.
  4. drop some cells in the reactor and off you go.

Operation

Normal Operation

  • Setting up the reactor for the run, avoiding contamination, etc.

Troubleshooting/Common problems

Bryan, anything here?

Temperature consistency in water bath

Masterflex tubing in the pump

avoid crushing this tubing by removing the clamp on the parastaltic pump when it is not running.

Contamination

Others?

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