Endy:Chemostat: Difference between revisions
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===Contamination=== | ===Contamination=== | ||
so basically after about three days the run is going to become contaminated. You will know that this happens because the OD will go crazy and diverge from steady state. somehow cells are getting in the top feed line and begin growing before getting to the reactor. I haven't really been able to figure out what is the best way to prevent this, perhaps running the reactor with a higher flow rate or finding a way to keep bubbles from forming and making their way up towards the feed line. Using minimal media might be the way to go here since it's less viscous yet this will prevent you from running as fast. | |||
Revision as of 15:49, 17 June 2006
This page is for storing information about operation of a bench-scale chemostat designed by Ben Kirkup. It is in development.
Design
- <photo>
- <CAD design> (ask ben if OK)
Setup
- Wash out chemostat. don't lose the little glass beads.
- autoclave making sure that all tubes are connected.
- setup everything and run media through the device for a little while. this is a good time to calculate the flow rate.
- drop some cells in the reactor and off you go.
Operation
Normal Operation
- Setting up the reactor for the run, avoiding contamination, etc.
Troubleshooting/Common problems
Bryan, anything here?
Temperature consistency in water bath
Masterflex tubing in the pump
avoid crushing this tubing by removing the clamp on the parastaltic pump when it is not running.
Contamination
so basically after about three days the run is going to become contaminated. You will know that this happens because the OD will go crazy and diverge from steady state. somehow cells are getting in the top feed line and begin growing before getting to the reactor. I haven't really been able to figure out what is the best way to prevent this, perhaps running the reactor with a higher flow rate or finding a way to keep bubbles from forming and making their way up towards the feed line. Using minimal media might be the way to go here since it's less viscous yet this will prevent you from running as fast.
