Endy:F2620

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Our work to develop and standardize cell-cell signaling technologies is described on the [http://parts2.mit.edu/registry/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling page] of the [http://parts.mit.edu/registry registry].
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__NOTOC__
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==Introduction==
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[[Image:F2620.de2.jpg|right|thumb|200px|Drew's datasheet]]
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We are working to comprehensively characterize a receiver device to enable cell-cell communication.  F2620 is a receiver device that responds to the presence of a signaling molecule in the extracellular media by activating transcription from a regulated promoter.  Our aims are to provide a well-characterized receiver device to the biological engineering community and secondly to develop a framework for device characterization that can be adapted to characterize other devices that can be used to build modular biological systems.  Further details about this work can be found on the [http://parts2.mit.edu/registry/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling page] of the [http://parts.mit.edu/registry registry].
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==Characterization data==
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{|{{Table}}
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!Experiment
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!Protocol
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!Data
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|-
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|[[Endy:F2620/Transfer Curve|Transfer Curve]]
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|[[Endy:F2620/Transfer Curve/Protocols|protocol]]
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|[[Endy:F2620/Transfer Curve/Data|data]]
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|-
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|[[Endy:F2620/Specificity|Specificity]]
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|[[Endy:F2620/Specificity/Protocols|protocol]]
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|[[Endy:F2620/Specificity/Data|data]]
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|-
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|[[Endy:F2620/Latency|Latency]]
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|[[Endy:F2620/Latency/Protocols|protocol]]
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|[[Endy:F2620/Latency/Data|data]]
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|-
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|[[Endy:F2620/Stability|Stability]]
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|[[Endy:F2620/Stability/Protocols|protocol]]
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|[[Endy:F2620/Stability/Data|data]]
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|}
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===F2620 Data Sheets===
 
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[[Image:F2620.de2.jpg|right|thumb|200px|Drew's datasheet]]
 
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[http://model.mit.edu/endipedia/images/7/7b/F2620.bc1.ppt Barry's first draft (.ppt file)]
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*[[Endy:F2620/Experiments Pending|Experiments Pending]]
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*[[Endy:F2620/Calibration|Calibration]]
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*[[Endy:F2620/Machine variation|Machine variation]]
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*[[Endy:F2620/Data Processing|Data Processing]]
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*[[Endy:F2620/Data/Archive|Old data plots and discussion of data-processing methods]]
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[http://model.mit.edu/endipedia/images/2/25/F2620.de2.ppt Drew's improved version (.ppt file)]
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==Communications==
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*[[Endy:F2620/Nature Biotech draft|Nature Biotech. draft]]
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*[[Endy:F2620/Figures]]
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*[[Nat Biotech Guidelines]]
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*[[Endy:F2620/Miscellaneous communications|Other communications]]
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[http://parts.mit.edu/registry/index.php/Part:BBa_F2620 F2620 Registry page]
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==F2620 Data Sheets==
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*[http://model.mit.edu/endipedia/images/7/7b/F2620.bc1.ppt Barry's first draft (.ppt file)]
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*[http://model.mit.edu/endipedia/images/2/25/F2620.de2.ppt Drew's improved version (.ppt file)]
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*[http://parts.mit.edu/registry/index.php/Part:BBa_F2620 F2620 Registry page]
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*[[Endy:F2620/Data sheet versions|Other data sheet versions]]
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===F2620 Paper===
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<math>
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*[[Endy:F2620:Draft1  | Draft]]
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P_{out} = \frac{P_{max}[3OC_6HSL]^n}{K^n+[3OC_6HSL]^n}
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</math>

Current revision

Introduction

Drew's datasheet
Drew's datasheet

We are working to comprehensively characterize a receiver device to enable cell-cell communication. F2620 is a receiver device that responds to the presence of a signaling molecule in the extracellular media by activating transcription from a regulated promoter. Our aims are to provide a well-characterized receiver device to the biological engineering community and secondly to develop a framework for device characterization that can be adapted to characterize other devices that can be used to build modular biological systems. Further details about this work can be found on the cell-cell signaling page of the registry.

Characterization data

Experiment Protocol Data
Transfer Curve protocol data
Specificity protocol data
Latency protocol data
Stability protocol data


Communications

F2620 Data Sheets


P_{out} = \frac{P_{max}[3OC_6HSL]^n}{K^n+[3OC_6HSL]^n}

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