Endy:Library-based construction
From OpenWetWare
(Difference between revisions)
Current revision (14:49, 17 July 2006) (view source) (→Procedure) |
|||
| (9 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | == | + | '''This is a work in progress, bear with us.''' |
| - | + | ||
| - | + | =="Fixing" several broken inverters== | |
| - | *[[ | + | We will demonstrate the utility of the [[PoPS device screening plasmid|screening plasmid]] for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method. |
| - | + | ||
| - | *[[ | + | ==Procedure== |
| + | [[Endy:Library-based construction/Inverter library protocol]] | ||
| + | |||
| + | ==Reshmaverters== | ||
| + | We are working with Reshma Shetty to test her new inverter designs. | ||
| + | *[[Knight:Evolving Reshmaverters]] - project page. | ||
| + | ===Results=== | ||
| + | |||
| + | |||
| + | ==TetR== | ||
| + | ===Results=== | ||
| + | Need to port the results described [[:Image:JK.SyntheticBiology2.0.Poster.pdf|here]]. | ||
| + | |||
| + | ==Mnt== | ||
| + | |||
| + | ==penI== | ||
| + | ===Results=== | ||
| + | |||
| + | ==Contact== | ||
| + | *[[Kelly Chang]] | ||
| + | *[[Jason Kelly]] | ||
Current revision
This is a work in progress, bear with us.
Contents |
"Fixing" several broken inverters
We will demonstrate the utility of the screening plasmid for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method.
Procedure
Endy:Library-based construction/Inverter library protocol
Reshmaverters
We are working with Reshma Shetty to test her new inverter designs.
- Knight:Evolving Reshmaverters - project page.
Results
TetR
Results
Need to port the results described here.
