Endy:MG1655(seq): Difference between revisions
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Back to [[Endy:E._coli_Strains| Endy lab E coli strains]] | |||
* '''Strain:''' MG1655(seq) | * '''Strain:''' MG1655(seq) | ||
* '''Genotype:''' See [http://cgsc.biology.yale.edu:80/cgi-bin/sybgw/cgsc/Strain/68076 CGSC#7740] | |||
* '''Phenotype:''' | |||
* '''Date:''' September 29, 2005 | * '''Date:''' September 29, 2005 | ||
* '''Experimenter:''' Francois St-Pierre | * '''Experimenter:''' Francois St-Pierre | ||
* '''Strain obtained from:''' E.coli Genetic Stock Center (CGSC). CGSC#7740 | * '''Strain obtained from:''' E.coli Genetic Stock Center (CGSC). CGSC#7740, Source person = M.S.Guyer (choice 1) | ||
* '''Stock preparation:''' The stock came as a few drops of a glycerolized culture placed on a filter disc. The filter disc was placed on a LB plate and ~200ul of LB was added on the filter to humidify it. A sterile loop (or toothpick), after touching the liquid, was used to streak on the same plate. The plate was incubated at 37C. A single colony was then grown in LB at 30C until it just reached late log phase - early saturation (OD600~2.3). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C. | * '''Stock preparation:''' The stock came as a few drops of a glycerolized culture placed on a filter disc. The filter disc was placed on a LB plate and ~200ul of LB was added on the filter to humidify it. A sterile loop (or toothpick), after touching the liquid, was used to streak on the same plate. The plate was incubated at 37C. A single colony was then grown in LB at 30C until it just reached late log phase - early saturation (OD600~2.3). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C. | ||
* '''Physical location of the stock:''' ( | * '''Physical location of the stock:''' | ||
** [[User:Stpierre|Francois St-Pierre]]'s stocks: 4 vials in box "Francois's Ecoli 1" (as of Oct3,2005). ID#: FSP B4 | |||
Latest revision as of 12:08, 3 October 2005
Back to Endy lab E coli strains
- Strain: MG1655(seq)
- Genotype: See CGSC#7740
- Phenotype:
- Date: September 29, 2005
- Experimenter: Francois St-Pierre
- Strain obtained from: E.coli Genetic Stock Center (CGSC). CGSC#7740, Source person = M.S.Guyer (choice 1)
- Stock preparation: The stock came as a few drops of a glycerolized culture placed on a filter disc. The filter disc was placed on a LB plate and ~200ul of LB was added on the filter to humidify it. A sterile loop (or toothpick), after touching the liquid, was used to streak on the same plate. The plate was incubated at 37C. A single colony was then grown in LB at 30C until it just reached late log phase - early saturation (OD600~2.3). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
- Physical location of the stock:
- Francois St-Pierre's stocks: 4 vials in box "Francois's Ecoli 1" (as of Oct3,2005). ID#: FSP B4
