Endy:MG1655(seq)

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• Strain: MG1655(seq)
• Date: September 29, 2005
• Experimenter: Francois St-Pierre
• Strain obtained from: E.coli Genetic Stock Center (CGSC). CGSC#7740
• Stock preparation: The stock came as a few drops of a glycerolized culture placed on a filter disc. The filter disc was placed on a LB plate and ~200ul of LB was added on the filter to humidify it. A sterile loop (or toothpick), after touching the liquid, was used to streak on the same plate. The plate was incubated at 37C. A single colony was then grown in LB at 30C until it just reached late log phase - early saturation (OD600~2.3). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
• Physical location of the stock: (boxes currently being organized)