Endy:MG1655: Difference between revisions

Revision as of 11:42, 3 October 2005

Strain: MG1655
Date: 09/2005
Experimenter: Francois St-Pierre
Strain obtained from: E.coli Genetic Stock Center (CGSC). CGSC#6300
Stock preparation: The stock came as a transport swab. The swab was dipped in 5mL of LB and grown at 37C. After ~1-2hours of growth, ampicillin (20ug/ml) and tetracycline (10ug/ml) were added. Growth was stopped when the culture reached OD600~2.9 (saturation). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
Physical location of the stock:
- Francois St-Pierre's stocks: 4 vials in box "Francois's Ecoli 1" (as of Oct3,2005);

@@ Line 6: / Line 6: @@
 * '''Experimenter:''' [[User:Stpierre|Francois St-Pierre]]
 * '''Strain obtained from:''' E.coli Genetic Stock Center (CGSC). CGSC#6300
-* '''Stock preparation:'''  The stock came as a few drops of a glycerolized culture placed on a filter disc. The filter disc was placed on a LB plate and ~200ul of LB was added on the filter to humidify it. A sterile loop (or toothpick), after touching the liquid, was used to streak on the same plate. The plate was incubated at 37C. A single colony was then grown in LB at 30C until it just reached saturation (OD600~3.1). Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
+* '''Stock preparation:'''  The stock came as a transport swab. The swab was dipped in 5mL of LB and grown at 37C. After ~1-2hours of growth, ampicillin (20ug/ml) and tetracycline (10ug/ml) were added. Growth was stopped when the culture reached OD600~2.9 (saturation).  Glycerol stocks were made by mixing 1ml of culture with 1ml of 40% glycerol, vortexing, and storing at -85C.
-* '''Physical location of the stock:''' (boxes currently being organized)
+* '''Physical location of the stock:'''
+** [[User:Stpierre|Francois St-Pierre]]'s stocks: 4 vials in box "Francois's Ecoli 1"  (as of Oct3,2005);