Endy:Making a long term stock of bacteria: Difference between revisions
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*While it is possible to make a long term stock from cells in stationary phase, | *While it is possible to make a long term stock from cells in stationary phase, ideally your culture should be in logarithmic growth phase. | ||
Latest revision as of 09:40, 29 September 2006
Back to Making a long term stock of bacteria
Materials
- 40% glycerol solution
- Cryogenic vials
Method
- Add 1 ml of 40% glycerol in H2O to a cryogenic vial.
- Add 1 ml sample from the culture of bacteria to be stored.
- Gently vortex the cryogenic vial to ensure the culture and glycerol is well-mixed.
- Alternatively, pipet to mix.
- Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
- On the side of the vial list all relevant information - part, vector, strain, date, researcher, etc.
- Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.
Notes
- While it is possible to make a long term stock from cells in stationary phase, ideally your culture should be in logarithmic growth phase.
