Endy:Making a long term stock of bacteria

Back to Making a long term stock of bacteria

Materials

• 40% glycerol solution
• Cryogenic vials

Method

• Add 1ml of 40% glycerol in H₂O to a cryongenic vial.
• Add 1ml sample from the culture of bacteria to be stored.
• Gently vortex the cryogenic vial to ensure the culture and glycerol is well mixed.
  • Alternatively, pipet to mix.
• Use a tough spot to put the name of the strain or some useful identifier on the top of the vial.
• On the side of the vial list all relevant information - part, vector, strain, date, researcher etc.
• Store in a freezer box in a -80C freezer. Remember to record where the vial is stored for fast retrieval later.

Notes

• While it is possible to make a long term stock from cells in stationary phase, optimally your culture should be in logarithmic growth phase.