Endy:Microfluidic Setup

This page describes the setup needed to operate a microfluidic chip via computer controlled pressure actuation. The setup described below is what we use in the Endy lab, and is adapted from the setup defined in Steve Quake's lab at Stanford.

Pressure Control

Pressure Guage (PSI Traix)

• digital pressure guage
• PG500-30-G
• I would probably find a normal, analog one instead, these have been a bit problematic -- the buttons often stick and they turn off after awhile to conserve batteries.

Microfluidics

Luer Stab Adapter (Clay Adams)

• 23 Guage
• Cost $1.5/each
• These look like needles for syringes, but have a blunt end. They are used to load the tubing with fluids. (you’ll also need standard syringes to go along with them).

Small Tubing

• Tubing, Tygon, 0.02 X .06", 100' (Cole Parmer)
• Reorder# 06418-02
• Cost $31.00 / roll (100ft)
• This tubing connects the manifold to the pins.

Large tubing

• You will need tubing to connect the pressure source to the manifolds. This will in part depend on your pressure source, but at the end you will need to reduce to tubing of size X (in order to interface with the manifold).

Pins

• Cut Tube, Type 304 (New England Small Tube Corp.)
• 23 Guage

Computer Control / Electronics

Digital I/O card for interfacing with BOB3 (National Instruments)

• Direct I/O to the BOB3 from the computer.
• PCI-DIO-32HS
• Jasonk 11:02, 28 July 2006 (EDT):Ty, was this specific to our computer, I forget how this played out.
• Will also need to get the cable.

BOB-3 Microfluidic Valve Manifold Controller (Fluidigm)

• Can operate 4 manifolds, so a total of 32 valves.

Miniature Solenoid valve cluster manifolds (Fluidigm)

• Only one type of manifold available, it contains 8 valves
• Each BOB-3 controller can interface with 4 manifolds (so total of 32 valves controlled).

Contact

Jason Kelly for more information.

Item name	Description	Company	Part #