Endy:NPE flow cytometer Calibration spheres

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We currently have spheres which can be used to:
We currently have spheres which can be used to:
-
(1) make sure the fluorescent channel(s) are behaving properly
+
#make sure the fluorescent channel(s) are behaving properly
-
(2) get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels  
+
#get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels  
-
(3) calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume
+
#calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume
The calibration we currently have (August 2005) were purchased from [http://www.spherotech.com Spherotech]. Other labs use spheres from [http://www.polysciences.com Polysciences] or [http://www.bangslabs.com Bangs Labs]. Michael Brochu (Sr, I believe) said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.
The calibration we currently have (August 2005) were purchased from [http://www.spherotech.com Spherotech]. Other labs use spheres from [http://www.polysciences.com Polysciences] or [http://www.bangslabs.com Bangs Labs]. Michael Brochu (Sr, I believe) said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.

Revision as of 09:40, 11 August 2005

We currently have spheres which can be used to:

  1. make sure the fluorescent channel(s) are behaving properly
  2. get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels
  3. calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume

The calibration we currently have (August 2005) were purchased from Spherotech. Other labs use spheres from Polysciences or Bangs Labs. Michael Brochu (Sr, I believe) said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.

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