Endy:NPE flow cytometer Calibration spheres: Difference between revisions
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#calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume | #calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume | ||
The calibration we currently have (August 2005) were purchased from [http://www.spherotech.com Spherotech]. Other labs use spheres from [http://www.polysciences.com Polysciences] or [http://www.bangslabs.com Bangs Labs]. Michael Brochu | The calibration we currently have (August 2005) were purchased from [http://www.spherotech.com Spherotech]. Other labs use spheres from [http://www.polysciences.com Polysciences] or [http://www.bangslabs.com Bangs Labs]. Michael Brochu Sr said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though. | ||
Revision as of 09:57, 16 August 2005
We currently have spheres which can be used to:
- make sure the fluorescent channel(s) are behaving properly
- get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels
- calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume
The calibration we currently have (August 2005) were purchased from Spherotech. Other labs use spheres from Polysciences or Bangs Labs. Michael Brochu Sr said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.
