Endy:NPE flow cytometer Calibration spheres

From OpenWetWare

Revision as of 23:32, 10 August 2005 by Barry Canton (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Add to My Links

We currently have spheres which can be used to: (1) make sure the fluorescent channel(s) are behaving properly (2) get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels (3) calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume

The calibration we currently have (August 2005) were purchased from Spherotech. Other labs use spheres from Polysciences or Bangs Labs. Michael Brochu (Sr, I believe) said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.

Personal tools