Endy:Notebook/Synthetic Biology Open Language

Project Description

• SBOL Visual is the graphical notation component of the Synthetic Biology Open Language (SBOL) being developed by the Synthetic Biology Data Exchange Group
  • It is intended for the formal specification of composite parts and devices.
  • It is targeted for use by biological engineers in forward engineering projects.
  • It encourages and supports model-driven engineering.
• SBOL Visual is an open standard.
  • Languages emerge from the friction of top-down control (eg. Preservation of the French Language) and bottom-up needs to communicate (eg. Pop Culture). With this in mind, SBOL Visual is flourishing in an environment of open communication while rigorously defining symbols, syntax, and grammar.
• SBOL Visual is an abstract tool for solving concrete problems.
  • Technical languages have an additional challenge of balancing the requirements of providing a philosophically consistent framework while still being useful for solving concrete problems. Balancing this tension is difficult and requires both top-down and bottom-up approaches. With that in mind, SBOL Visual has a rigorous and consistent structure and is being used in concrete software tools while working on concrete Synthetic Biology projects.

Developers and Contributors

• Lead Developers
  • Suzie Bartram
  • Drew Endy
  • Anusuya Ramasubramanian
  • Cesar Rodriguez
• Contributors
  • Lesia Bilitchenko, Jerome Bonnet, Barry Canton, Deepak Chandran, Mackenzie Cowell, Douglas Densmore, Joanna Chen, Eric Fernandez, Tim Ham, Raik Gruenberg, Jason Kelly, Adam Liu, Richard Mar, Lance Martin, Alec Nielsen, Robert Ovadia, Randy Rettberg, Herbert Sauro, Reshma Shetty, Francois St-Pierre, Emma Weeding, and Ming Yan.

Review

Survey of Synthetic Biology Diagrams

• http://parts.mit.edu/wiki/index.php/IAP2004:Polkadorks

Graphical Notation Standards/Software Tools

• Systems Biology Grahical Notation
• EcoCyc Notation
• Diagrammatic Cell Language
• GenoCAD
• BioTapestry
• CellDesigner
• SynBioSS

DNA Sequence Features

• Registry of Standard Biological Parts
• GenBank
• Sequence Ontology

Literature

• Novere, et al. Systems Biology Graphical Notation. Nature Biotechnology 2009 vol. 27 (8) pp. 735-41 Link
• Matsuoka, et al. Consistent design schematics for biological systems: standardization of representation in biological engineering. Journal of the Royal Society Interface. EPub June 3 2009 Link
• Shetty, et al. Engineering BioBrick vectors from BioBrick parts. Journal of Biological Engineering. 2008; 2: 5. Link
• Voigt. Genetic parts to program bacteria. Current Opinion in Biotechnology, 17(5):548–57, Oct 2006. Link
• Hill et al. SynBioSS: The Synthetic Biology Modeling Software Suite. Bioinformatics 24 (21): 2551. (2008) Link
• Andrianantoandro, et al. Synthetic biology: new engineering rules for an emerging discipline. Molecular Systems Biology. 2006 vol. 2 pp. 2006.0028.
• Endy. Foundations for engineering biology. Nature. 2005 vol. 438 (7067) pp. 449-53.

Use Cases, Requirements and Comments

Click here to read the ongoing discussions... You are welcome to contribute!

Design

Basic Parts

• Upcoming Enhancements
  • The symbols will be provided with and without the abstract DNA Strand
  • A subset of the symbols will be provided with color
    • Green Promoters
    • Red Terminators

Central Dogma

Symbol	Name & Description	Status	Proposed By
	Origin of Replication The circle represents a plasmid.  The rectangle indicates the site of the replication origin on the plasmid.  An indication of the plasmid copy number is OPTIONAL but MUST be located in the center of the circle.  Molecules per cell is the RECOMMENDED scale.	Included in v. 1.0	Bartram Endy
	Shorthand Origin of Replication Shorthand version of the Origin of Replication.  The rectangle is omitted. This symbol SHOULD be used for rapid drawing and communication. However, the Origin of Replication symbol is RECOMMENDED.	Included in v. 1.0	Bartram Endy
	Forward Constitutive Promoter Represents a DNA sequence that promotes RNA polymerase binding and transcription in the forward strand.  The open square indicates constitutive transcription.	Included in v. 1.0	Bartram Endy
	Reverse Constitutive Promoter Represents a constitutive promoter initiating transcription in the reverse strand.	Included in v. 1.0	Bartram Endy
	Forward Inducible Promoter Represents a promoter that requires induction before promoting transcription in the forward strand.  The "+" symbol in the square indicates that the promoter is inducible.	Included in v. 1.0	Bartram Endy
	Reverse Inducible Promoter Represents a promoter that requires induction before promoting transcription in the reverse strand.  The "+" symbol in the square indicates that the promoter is inducible.	Included in v. 1.0	Bartram Endy
	Forward Repressible Promoter Represents a promoter where transcription in the forward strand can be repressed.  The "-" symbol in the square indicates that the promoter is repressible.	Included in v. 1.0	Bartram Endy
	Reverse Repressible Promoter Represents a promoter where transcription in the reverse strand can be repressed. The "-" symbol in the square indicates that the promoter is repressible.	Included in v. 1.0	Bartram Endy
	Forward Promoter with Downstream Operator Represents a forward promoter with a downstream operator.  The OPTIONAL "+" symbol MUST be located in the square and indicates an inducible promoter.  The OPTIONAL "-" symbol MUST be located in the square and indicates a repressible promoter.  The rectangle to the right of the square indicates a downstream operator.  Information about the specific location and function of the operator is OPTIONAL, but MUST be located within the rectangle.	Included in v. 1.0	Bartram Endy
	Forward Promoter with Upstream Operator Represents a forward promoter with an upstream operator.  The OPTIONAL "+" symbol MUST be located in the square and indicates an inducible promoter.  The OPTIONAL "-" symbol MUST be located in the square and indicates a repressible promoter.  The rectangle to the left of the square indicates an upstream operator.  Information about the specific location and function of the operator is OPTIONAL, but MUST be located within the open rectangle.	Included in v. 1.0	Bartram Endy
	Forward Promoter with Upstream and Downstream Operators Represents a forward promoter with upstream and downstream operators.  The OPTIONAL "+" symbol MUST be located in the square and indicates an inducible promoter.  The OPTIONAL "-" symbol MUST be located in the square and indicates a repressible promoter.  The rectangles to the left and right of the square indicate upstream and downstream operators, respectively.  Information about the specific location and function of the operators are OPTIONAL, but MUST be located within the rectangles.	Included in v. 1.0	Bartram Endy
	Forward Open Reading Frame Represents a forward open reading frame (ORF).  The polygon indicates the single continuous DNA fragment of the ORF.  The angled right wall indicates the forward direction of the reading frame.	Included in v. 1.0	Common Use
	Reverse Open Reading Frame Represents a reverse open reading frame (ORF).  The polygon indicates the single continuous DNA fragment of the ORF.  The angled left wall indicates the reverse direction of the reading frame.	Included in v. 1.0	Common Use
	Forward Translation Start Site Represents a Shine-Delgarno sequence in the forward strand of prokaryotes, IRES in viruses and ribosome loading structure in eukaryotes.	Included in v. 1.0	Ham
	Reverse Translation Start Site Represents a Shine-Delgarno sequence on the reverse strand in prokaryotes and equivalent sites or structures in eukaryotes.	Included in v. 1.0	Ham
	Forward Terminator Represents the location on a DNA sequence where transcription ends in the forward strand.	Included in v. 1.0	Bonnet Fernandez
	Reverse Terminator Represents the location on a DNA sequence where transcription ends in the reverse strand.	Included in v. 1.0	Bonnet
	Bidirectional Terminator Represents the location on a DNA sequence where transcription ends in both strands.	Included in v. 1.0	Bonnet
	Ribonuclease Site Represents the location on a DNA sequence that codes for a ribonuclease cleavage site on the RNA.  The dashed line indicates a functional element located on the RNA. The "x" symbol indicates cleavage of the RNA.	Included in v. 1.0	Bartram Endy
	RNA Stability Element, "Stable"  Represents a location on a DNA sequence that codes for an RNA secondary structure that effects the stability of the RNA.  The dashed line indicates a functional element located on the RNA.  The diameter of the circle SHOULD indicate the half life of the RNA.  A log scale is RECOMMENDED.  The larger circle indicates qualitatively an RNA molecule with a long half-life.	Included in v. 1.0	Bartram Endy
	RNA Stability Element, "Intermediate" Represents a location on a DNA sequence that codes for an RNA secondary structure that effects the stability of the RNA.  The dashed line indicates a functional element located on the RNA.  The diameter of the circle SHOULD indicate the half life of the RNA.  A log scale is RECOMMENDED.  The smaller circle indicates qualitatively an RNA molecule with an intermediate half-life.	Included in v. 1.0	Bartram Endy
	RNA Stability Element, "Unstable" Represents the location on a DNA sequence that codes for an RNA secondary structure that effects the stability of the RNA.  The dashed line indicates a functional element located on the RNA.  The diameter of the circle SHOULD indicate the half-life of the RNA.  A log scale is RECOMMENDED.  The smallest circle indicates qualitatively an RNA molecule with a short life span.	Included in v. 1.0	Bartram Endy
	Protease Site Represents the location on a DNA sequence that codes for a protease cleavage site.  The solid line indicates a functional element located on a peptide/protein.  The "x" symbol indicates cleavage of a peptide or protein.	Included in v. 1.0	Bartram Endy
	Protein Degradation Element "Stable"  Represents a location on a DNA sequence that codes for a degradation-related structure in a peptide/protein .  The solid line indicates a functional element located on the peptide/protein.  The diameter of the circle SHOULD indicate the half-life of the peptide/protein.  A log scale is RECOMMENDED.  The larger circle indicates qualitatively a peptide/protein with a long half-life.	Included in v. 1.0	Bartram Endy
	Protein Degradation Element "Intermediate" Represents a location on a DNA sequence that codes for a degradation-related structure in a peptide/protein .  The solid line indicates a functional element located on the peptide/protein.  The diameter of the circle SHOULD indicate the half-life of the peptide/protein.  A log scale is RECOMMENDED.  The smaller circle indicates qualitatively a peptide/protein with an intermediate half-life.	Included in v. 1.0	Bartram Endy
	Protein Degradation Element "Unstable" Represents a location on a DNA sequence that codes for a degradation-related structure in a peptide/protein .  The solid line indicates a functional element located on the peptide/protein.  The diameter of the circle SHOULD indicate the half-life of the peptide/protein.  A log scale is RECOMMENDED.  The smallest circle indicates qualitatively a peptide/protein with a short half-life.	Included in v. 1.0	Bartram Endy

Genetic Engineering

Symbol	Name & Description	Status	Proposed By
	Forward Primer Site Represents a site for primer binding in the forward strand.	Included in v. 1.0	Common Use
	Reverse Primer Site Represents a site for primer binding in the reverse strand.	Included in v. 1.0	Common Use
	Forward Barcode Represents a location on a DNA sequence where developer information is encoded on the forward strand.  The encoded information can, for example, include the name of the individual or team that developed the standard biological part.	Included in v. 1.0	Bartram Endy
	Reverse Barcode Represents a location on a DNA sequence where developer information is encoded on the reverse strand.  The encoded information can, for example, include the name of the individual or team that developed the standard biological part.	Included in v. 1.0	Bartram Endy
	Prefix Represents the prefix sequence of an assembly standard.  The identifier of the specific prefix sequence used is OPTIONAL, but it MUST be located in the rectangle.	Included in v. 1.0	Bartram Endy
	Shorthand Prefix Represents the prefix sequence of an assembly standard.  The rectangle found in the Prefix symbol is excluded.	Included in v. 1.0	Bartram Endy
	Suffix Represents the suffix sequence of an assembly standard.  The identifier of the specific prefix sequence used is OPTIONAL, but it MUST be located in the rectangle.	Included in v. 1.0	Bartram Endy
	Shorthand Suffix Represents the prefix sequence of an assembly standard.  The rectangle found in the Suffix symbol is excluded.	Included in v. 1.0	Bartram Endy
	Scar  Represents where a suffix and prefix sequence were ligated.	Included in v. 1.0	Bartram Endy
	3' Overhang Restriction Site Represents a restriction endonuclease site that results in 3' overhangs.  The diagonal line indicates the geometry of the cut site and the 3' sticky ends.	Included in v. 1.0	Bartram Endy
	5' Overhang Restriction Site Represents a restriction endonuclease site that results in 5' overhangs.  The diagonal line indicates the geometry of the cut site and the 5' sticky ends.	Included in v. 1.0	Bartram Endy
	Blunt Restriction Site Represents a restriction endonuclease site that results in no overhangs.  The straight line indicates the geometry of the cut site.	Included in v. 1.0	Gruenberg
	Insulator Coming Soon...	Under Development	Endy Rodriguez

Devices

Grammar

Ideas for Symbols from SBOL semantic Terminology

(needs to be converted to wiki table)

Implementation

• Symbols
  • Version 1.0
    • Download
    • Released on 11-18-2009
    • PDF Vector Images
    • 72 & 300 dpi PNGs
  • Prior Versions
• Software Tools
  • TinkerCell
  • Clotho-Spectacles
  • OmniGraffle
• Source Code
  • Link
• Tutorials
  • Incorporating Symbols into PowerPoint 2004 for Macs
  • Incorporating Symbols into PowerPoint 2007 for Windows
• Specifications
  • RFC 16
  • RFC 18

Maintenance

Symbol Status

Deprecated Symbols

Notes

• Tiny URL
  • http://tinyurl.com/sbolv

Recent changes