Endy:PCR Supermix
From OpenWetWare
For a 100µL reaction using the supermix:
- 100µL PCR Supermix
- 1.5µL forward primer (40 mM)
- 1.5µL reverse primer (40 mM)
- 0.5µL template DNA (10ng/µL)
Protocol:
- 95 deg - 5 min
- 95 deg - 30s
- 55 deg (or appropriate Tm) - 30s
- 68 deg - 1min/kbp of template
- Repeat steps 2-4 35x
- 68 deg - 10 min
- Store at 4 deg