Endy:STN Chemostat Design: Difference between revisions

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Contact [[Caitlin Conboy]], [[Jennifer Braff]]
Contact [[Caitlin Conboy]], [[Jennifer Braff]].


==Schematic==
This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.
 
==Design Images==
[[Image:STN Chemostat Schematic.png|left|240px|thumb|'''Simple schematic for a single chemostat chamber.''']]
[[Image:STN Chemostat Schematic.png|left|240px|thumb|'''Simple schematic for a single chemostat chamber.''']]
[[Image:STN Chemostat Photo.png|left|160px|thumb|'''Photo of a six-chamber chemostat.''' Built by [[Caitlin Conboy|cmc]] and [[Jennifer Braff|jcb]].]]
[[Image:STN Chemostat Photo.png|left|160px|thumb|'''Photo of a six-chamber chemostat.''' Built by [[Caitlin Conboy|cmc]] and [[Jennifer Braff|jcb]].]]
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==Components==
==Components==
''This page is in progress''
===Equipment===
*Circulating Water Bath
**VWR
*Peristaltic pump
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''
*Tube Rack (to hold the chemostat chambers upright in the water bath)
**USA Scientific
===Glassware===
*1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
*Rubber caps for 1L flasks with two drilled holes
*Glass tubing
*25mm glass test tubes, one per chemostat chamber


===Tubing===
*Silicone tubing
*Connectors
 
==Assembly==
==Assembly==



Revision as of 11:52, 20 June 2006

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Design Images

Simple schematic for a single chemostat chamber.
Photo of a six-chamber chemostat. Built by cmc and jcb.


Components

This page is in progress

Equipment

  • Circulating Water Bath
    • VWR
  • Peristaltic pump
  • Tube Rack (to hold the chemostat chambers upright in the water bath)
    • USA Scientific

Glassware

  • 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
  • Rubber caps for 1L flasks with two drilled holes
  • Glass tubing
  • 25mm glass test tubes, one per chemostat chamber

Tubing

  • Silicone tubing
  • Connectors

Assembly

Operation

See Endy:STN Chemostat Protocol