Endy:STN Chemostat Design

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*Peristaltic pump
*Peristaltic pump
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''  
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''  
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*Bubbler
*Tube Rack (to hold the chemostat chambers upright in the water bath)
*Tube Rack (to hold the chemostat chambers upright in the water bath)
**USA Scientific
**USA Scientific
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*Silicone tubing
*Silicone tubing
*Connectors
*Connectors
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+
 
==Assembly==
==Assembly==

Revision as of 14:19, 20 June 2006

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Contents

Design Images

Simple schematic for a single chemostat chamber.
Simple schematic for a single chemostat chamber.
Photo of a six-chamber chemostat. Built by cmc and jcb.
Photo of a six-chamber chemostat. Built by cmc and jcb.


Components

This page is in progress

Equipment

  • Circulating Water Bath
    • VWR
  • Peristaltic pump
  • Bubbler
  • Tube Rack (to hold the chemostat chambers upright in the water bath)
    • USA Scientific

Glassware

  • 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
  • Rubber caps for 1L flasks with two drilled holes
  • Glass tubing
  • 25mm glass test tubes, one per chemostat chamber

Tubing

  • Silicone tubing
  • Connectors

Assembly

Operation

See Endy:STN Chemostat Protocol

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