Endy:STN Chemostat Design

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (14:36, 20 June 2006) (view source)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
Contact [[Caitlin Conboy]], [[Jennifer Braff]]
+
Contact [[Caitlin Conboy]], [[Jennifer Braff]].
-
==Schematic==
+
This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.
-
[[Image:STN Chemostat Schematic.png|left|300px|thumb|'''Simple schematic for a single chemostat chamber.''']]
+
 
 +
Also see [[Endy:Chemostat]] for an alternative chemostat chamber design and additional info on running chemostats.
 +
 
 +
==Design Images==
 +
[[Image:STN Chemostat Schematic.png|left|240px|thumb|'''Simple schematic for a single chemostat chamber.''']]
 +
[[Image:STN Chemostat Photo.png|left|160px|thumb|'''Photo of a six-chamber chemostat.''' Built by [[Caitlin Conboy|cmc]] and [[Jennifer Braff|jcb]].]]
 +
<br style="clear:both" />
==Components==
==Components==
 +
''This page is in progress''
 +
===Equipment===
 +
*Circulating Water Bath
 +
**VWR
 +
*Peristaltic pump
 +
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''
 +
*Bubbler (Acquarium bubbler)
 +
 +
===Glassware===
 +
*1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
 +
*Rubber caps for 1L flasks with two drilled holes
 +
*Glass tubing
 +
*25mm glass test tubes, one per chemostat chamber
 +
 +
===Tubing, Connectors===
 +
*Silicone tubing
 +
*Connectors, ''various, specify''
 +
*Three-way stopcock
 +
**Cole Parmer luer fittings, chrome-plated brass three-way stopcock
 +
 +
===Other===
 +
*Needles, 2 per chamber
 +
**18 gauge deflected point non-coring septum penetration needles (Popper).
 +
*Tube Rack (to hold the chemostat chambers upright in the water bath)
 +
**USA Scientific
 +
*Syringes, to innoculate and sample from chemostat
==Assembly==
==Assembly==

Current revision

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.

Contents

Design Images

Simple schematic for a single chemostat chamber.
Simple schematic for a single chemostat chamber.
Photo of a six-chamber chemostat. Built by cmc and jcb.
Photo of a six-chamber chemostat. Built by cmc and jcb.


Components

This page is in progress

Equipment

  • Circulating Water Bath
    • VWR
  • Peristaltic pump
  • Bubbler (Acquarium bubbler)

Glassware

  • 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
  • Rubber caps for 1L flasks with two drilled holes
  • Glass tubing
  • 25mm glass test tubes, one per chemostat chamber

Tubing, Connectors

  • Silicone tubing
  • Connectors, various, specify
  • Three-way stopcock
    • Cole Parmer luer fittings, chrome-plated brass three-way stopcock

Other

  • Needles, 2 per chamber
    • 18 gauge deflected point non-coring septum penetration needles (Popper).
  • Tube Rack (to hold the chemostat chambers upright in the water bath)
    • USA Scientific
  • Syringes, to innoculate and sample from chemostat

Assembly

Operation

See Endy:STN Chemostat Protocol

Personal tools