Endy:STN Chemostat Design
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This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable. | This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable. | ||
| + | |||
| + | Also see [[Endy:Chemostat]] for an alternative chemostat chamber design and additional info on running chemostats. | ||
==Design Images== | ==Design Images== | ||
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*Peristaltic pump | *Peristaltic pump | ||
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?'' | **[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?'' | ||
| - | * | + | *Bubbler (Acquarium bubbler) |
| - | + | ||
===Glassware=== | ===Glassware=== | ||
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*25mm glass test tubes, one per chemostat chamber | *25mm glass test tubes, one per chemostat chamber | ||
| - | ===Tubing=== | + | ===Tubing, Connectors=== |
*Silicone tubing | *Silicone tubing | ||
| - | *Connectors | + | *Connectors, ''various, specify'' |
| - | + | *Three-way stopcock | |
| + | **Cole Parmer luer fittings, chrome-plated brass three-way stopcock | ||
| + | |||
| + | ===Other=== | ||
| + | *Needles, 2 per chamber | ||
| + | **18 gauge deflected point non-coring septum penetration needles (Popper). | ||
| + | *Tube Rack (to hold the chemostat chambers upright in the water bath) | ||
| + | **USA Scientific | ||
| + | *Syringes, to innoculate and sample from chemostat | ||
| + | |||
==Assembly== | ==Assembly== | ||
Current revision
Contact Caitlin Conboy, Jennifer Braff.
This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.
Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.
Contents |
Design Images
Components
This page is in progress
Equipment
- Circulating Water Bath
- VWR
- Peristaltic pump
- L/S peristaltic pump from Cole Parmer. Which pump, specifically?
- Bubbler (Acquarium bubbler)
Glassware
- 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
- Rubber caps for 1L flasks with two drilled holes
- Glass tubing
- 25mm glass test tubes, one per chemostat chamber
Tubing, Connectors
- Silicone tubing
- Connectors, various, specify
- Three-way stopcock
- Cole Parmer luer fittings, chrome-plated brass three-way stopcock
Other
- Needles, 2 per chamber
- 18 gauge deflected point non-coring septum penetration needles (Popper).
- Tube Rack (to hold the chemostat chambers upright in the water bath)
- USA Scientific
- Syringes, to innoculate and sample from chemostat
