Endy:STN Chemostat Design

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Current revision (14:36, 20 June 2006) (view source)
 
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*Peristaltic pump
*Peristaltic pump
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''  
**[http://www.coleparmer.com/catalog/Product_index.asp?cls=4440|Masterflex L/S peristaltic pump] from Cole Parmer. ''Which pump, specifically?''  
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*Bubbler
+
*Bubbler (Acquarium bubbler)
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*Tube Rack (to hold the chemostat chambers upright in the water bath)
+
-
**USA Scientific
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===Glassware===
===Glassware===
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*25mm glass test tubes, one per chemostat chamber
*25mm glass test tubes, one per chemostat chamber
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===Tubing===
+
===Tubing, Connectors===
*Silicone tubing
*Silicone tubing
-
*Connectors
+
*Connectors, ''various, specify''
 +
*Three-way stopcock
 +
**Cole Parmer luer fittings, chrome-plated brass three-way stopcock
 +
 
 +
===Other===
 +
*Needles, 2 per chamber
 +
**18 gauge deflected point non-coring septum penetration needles (Popper).
 +
*Tube Rack (to hold the chemostat chambers upright in the water bath)
 +
**USA Scientific
 +
*Syringes, to innoculate and sample from chemostat
==Assembly==
==Assembly==

Current revision

Contact Caitlin Conboy, Jennifer Braff.

This is a flexible design for building a chemostat from off-the-shelf parts. The number of chemostat chambers and the culture volume per chamber are adjustable.

Also see Endy:Chemostat for an alternative chemostat chamber design and additional info on running chemostats.

Contents

Design Images

Simple schematic for a single chemostat chamber.
Simple schematic for a single chemostat chamber.
Photo of a six-chamber chemostat. Built by cmc and jcb.
Photo of a six-chamber chemostat. Built by cmc and jcb.


Components

This page is in progress

Equipment

  • Circulating Water Bath
    • VWR
  • Peristaltic pump
  • Bubbler (Acquarium bubbler)

Glassware

  • 1L flasks. Need one per type of media to be used in the chemostat, plus one or more to collect the effluent.
  • Rubber caps for 1L flasks with two drilled holes
  • Glass tubing
  • 25mm glass test tubes, one per chemostat chamber

Tubing, Connectors

  • Silicone tubing
  • Connectors, various, specify
  • Three-way stopcock
    • Cole Parmer luer fittings, chrome-plated brass three-way stopcock

Other

  • Needles, 2 per chamber
    • 18 gauge deflected point non-coring septum penetration needles (Popper).
  • Tube Rack (to hold the chemostat chambers upright in the water bath)
    • USA Scientific
  • Syringes, to innoculate and sample from chemostat

Assembly

Operation

See Endy:STN Chemostat Protocol

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