Endy:Screening plasmid: Difference between revisions
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'''This page is a work in progress.''' | '''This page is a work in progress.''' | ||
==Introduction== | |||
[[Image:ScreeningPlasmidSchematic.PNG|400px|left|thumb|Schematic of the screening plasmid design.]] | [[Image:ScreeningPlasmidSchematic.PNG|400px|left|thumb|Schematic of the screening plasmid design.]] | ||
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The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement. | The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement. | ||
==Screening Plasmid 1.0== | |||
[[Image:ScreeningPlasmid1.0.PNG|400px|left|thumb|design of Screening Plasmid 1.0]] | |||
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*[[Endy:Screening plasmid background|Background]] | *[[Endy:Screening plasmid background|Background]] | ||
*[[Endy:Screening plasmid protocols|Protocols]] | *[[Endy:Screening plasmid protocols|Protocols]] | ||
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*[[Endy:Screening plasmid constructs|Constructs]] | *[[Endy:Screening plasmid constructs|Constructs]] | ||
==Terminator Characterization== | ===Terminator Characterization via Screening Plasmid 1.0=== | ||
*[[Endy:Terminator characterization/Protocols|Protocols]] | *[[Endy:Terminator characterization/Protocols|Protocols]] | ||
*[[Endy:Terminator characterization/Analysis|Analysis]] | *[[Endy:Terminator characterization/Analysis|Analysis]] | ||
==Inverter Characterization== | ===Inverter Characterization via Screening Plasmid 1.0=== | ||
[[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve. | [[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve. | ||
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*[[Endy:Inverter characterization/Protocols|Protocols]] | *[[Endy:Inverter characterization/Protocols|Protocols]] | ||
*[[Endy:Screening plasmid inverters|Inverters]] | *[[Endy:Screening plasmid inverters|Inverters]] | ||
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Revision as of 19:24, 9 May 2006
This page is a work in progress.
Introduction
The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement.
Screening Plasmid 1.0
Terminator Characterization via Screening Plasmid 1.0
Inverter Characterization via Screening Plasmid 1.0
