Endy:Screening plasmid: Difference between revisions
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===Terminator Characterization via Screening Plasmid 1.0=== | ===Terminator Characterization via Screening Plasmid 1.0=== | ||
[[Image: | [[Image:TermDotPLot.JPG|left|300px|thumb|'''Characterization of 6 terminators from the Registry of Standard Biological Parts''' inserted into the Screening Plasmid. The black line is the best fit to the empty screening plasmid, and serves as a standard for 0% termination efficiency. Functional terminators should lie below the line, note that B0025 (red) is sometimes acting as a promoter.]] | ||
[[Image:TermsCorrected.jpg|left|300px|thumb|Histogram of calculated termination efficiencies for each terminator. Note that B0025 is mostly off scale.]] | |||
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*[[Endy:Terminator characterization/Protocols|Protocols]] | *[[Endy:Terminator characterization/Protocols|Protocols]] | ||
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===Inverter Characterization via Screening Plasmid 1.0=== | ===Inverter Characterization via Screening Plasmid 1.0=== | ||
[[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve. | [[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve.]] | ||
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*[[Endy:Inverter characterization/Protocols|Protocols]] | *[[Endy:Inverter characterization/Protocols|Protocols]] |
Revision as of 17:33, 18 May 2006
This page is a work in progress.
Introduction
The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement.
Screening Plasmid 1.0
Terminator Characterization via Screening Plasmid 1.0
Inverter Characterization via Screening Plasmid 1.0
Screening Plasmid 2.0
We are in the process of designing a new version of the screening plasmid to account for some of the shortcomings of the previous version. An early list of possible improvements:
- Brighter FPs (in particular RFP is very dim)
- Lower copy plasmid
References
[2] Khlebnikov et al,Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
[3] Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon. Biotechnol Bioeng. 2002 Dec 30;80(7):762-76.