Endy:Screening plasmid: Difference between revisions
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[[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve.]] | [[Image:Q04740sp.PNG|thumb|400px|left|'''Characterization of Q04740: '''Dot plot of one replicate is shown in upper right. Mean RFP expression for 3 replicates is plotted against GFP showing characteristic inverter transfer curve.]] | ||
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*[[Endy:Screening plasmid/Inverter characterization/Protocols | *[[Endy:Screening plasmid/Inverter characterization/Protocols|Protocols]] | ||
*[[Endy:Screening plasmid/Inverter characterization | *[[Endy:Screening plasmid/Inverter characterization|Inverters]] | ||
*[[Endy:Screening plasmid/Inverter characterization/Analysis | *[[Endy:Screening plasmid/Inverter characterization/Analysis|Analysis]] | ||
==Screening Plasmid 2.0== | ==Screening Plasmid 2.0== |
Revision as of 19:51, 30 June 2006
This page is a work in progress.
Introduction
The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement.
Screening Plasmid 1.0
Terminator Characterization via Screening Plasmid 1.0
Inverter Characterization via Screening Plasmid 1.0
Screening Plasmid 2.0
We are in the process of designing a new version of the screening plasmid to account for some of the shortcomings of the previous version. Details can be found here:
References
[2] Khlebnikov et al,Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
[3] Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon. Biotechnol Bioeng. 2002 Dec 30;80(7):762-76.